LOCUS       sequence-161447-         477 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for conjugational transfer into cyanobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Kukil K, Lindberg P
  TITLE     Expression of phenylalanine ammonia lyases in Synechocystis sp. PCC 
            6803 and subsequent improvements of sustainable production of 
            phenylpropanoids.
  JOURNAL   Microb Cell Fact. 2022 Jan 10;21(1):8. doi: 
            10.1186/s12934-021-01735-8.
  PUBMED    35012528
REFERENCE   2  (bases 1 to 477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2022-01-10"; volume: "21(1):8. doi"; pages: " 
            10.1186/s12934-021-01735-8"
COMMENT     Sequence Label: pEEK1
FEATURES             Location/Qualifiers
     source          1..477
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             436..459
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc
       61 ttttataggt gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag
      121 ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag
      181 cgttcggcac gcagacgacg ggcttcattc tgcatggttg cgcttaccag accggagata
      241 ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac
      301 gctgcttcat agcatacctc tttttgacat acttcgggta tacatatcag tatatattct
      361 tataccgcaa aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccaga
      421 tctggtagcg gaagttggag tcatcctcag ttcgagaagt aaactagtta ccgcggc
//
