LOCUS       sequence-161449-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for conjugational transfer into cyanobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Miao R, Liu X, Englund E, Lindberg P, Lindblad P
  TITLE     Isobutanol production in Synechocystis PCC 6803 using heterologous 
            and endogenous alcohol dehydrogenases.
  JOURNAL   Metab Eng Commun. 2017 Jul 29;5:45-53. doi: 
            10.1016/j.meteno.2017.07.003. eCollection 2017 Dec.
  PUBMED    29188183
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2017-07-29"; volume: "5:45-53. doi"; pages: " 
            10.1016/j.meteno.2017.07.003. eCollection 2017 Dec"
COMMENT     Sequence Label: pEEK2
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      469..540
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      556..583
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      596..653
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(677..694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca
       61 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg
      121 ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc
      181 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgcg
      241 cttaccagac cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt
      301 caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata
      361 catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct
      421 ggcttttagt aagcctctag atgcttacta gttaccgcgg ccaggcatca aataaaacga
      481 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      541 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatac tgcagtccgg
      601 caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc
      661 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      721 cggtatcagc
//
