LOCUS       sequence-161452-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of exogenous SL1 in multiple tissues of C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Ma X, Zhan G, Sleumer MC, Chen S, Liu W, Zhang MQ, Liu X
  TITLE     Analysis of C. elegans muscle transcriptome using 
            trans-splicing-based RNA tagging (SRT).
  JOURNAL   Nucleic Acids Res. 2016 Aug 23. pii: gkw734.
  PUBMED    27557708
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 23. pii: gkw734."
COMMENT     Sequence Label: Pu2-3::tag::sl1(G16C)
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(677..693)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(678..694)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(730..746)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(730..746)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(743..765)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    754..770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 agctgccctt cgcgcccggg cactaaaggg aacaaaagct ggagctccac cgcggtggcg
       61 gccgctctag aggactccgg cttcagcacg actccagacg acgccaaaga gcccgaaaaa
      121 gttgcagcta attaataacg tttccccaac agtttctccc cccgattaca cgtgtaaaaa
      181 tcctatggac tttcgaaatt attcacttca ccgagttcta atatttaatt tattgatttt
      241 tctttgaact tttcatattt taattaattt attatataaa gatttttaat taattcaaag
      301 acatacctaa ttgtctttct tttctaggcc acgaaacaaa gagtgctcca cgcgcaccca
      361 cgagttgtcg gtacacagct ccccactacc cctaccctct cctccattca gtacacgacg
      421 ctcttccgat ctggtttaat tacccaactt tgaggtaaac attgaaactg acccaaagaa
      481 atttggcgtt agctataaat tttggaacgt ctcctctcgg ggagacaaaa atactaagaa
      541 attcgaaaat cggttcatcc atgttggaga acggtccatt cgaaaatatg tggagatgag
      601 ggagcatggc tgtccgtatg tggagatgtg ggaaggtgat gatattattg gggaacgaat
      661 tcgatatcaa gcttatcgat accgtcgacc tcgaggccta ggcgcgaagg gcagcttggc
      721 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      781 catacgagcc g
//
