LOCUS       sequence-161456-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged OSBP PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Levine TP, Munro S
  TITLE     The pleckstrin homology domain of oxysterol-binding protein 
            recognises a determinant specific to Golgi membranes.
  JOURNAL   Curr Biol. 1998 Jun 18;8(13):729-39.
  PUBMED    9651677
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "1998-06-18"; volume: "8(13)"; pages: "729-39"
COMMENT     Sequence Label: pRS406-PHO5-GFP-OSBP PH domain
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(322..351)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(404..424)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cggttatcac gaattcttct tcacagcttt ggccttggcc agttccaggg ccgtcaccca
       61 gcgctgccgc tcaacttctg aactagcttt cagatggtag gtctgagcac ccccattgga
      121 aatgatgaag ttgcaggagt cctccacggt gatgttggct gtggcgaggt tgatggtacc
      181 acggcaggta tgtctcatct ctgcctttga tctgtagtag ctcaggagcc cgttgctcag
      241 cacgaaccat cgccgctggt agcctttgat ataattggtc catttgaaga gccagccctc
      301 tcgagccgag cccgaagatc tcaagtcctc ttcagaaatg agcttttgct ccatggatcc
      361 aagcttgtat agttcatcca tgccatgtgt aatcccagca gctgttacaa actcaagaag
      421 gaccatgtgg tctctctttt cgttgggatc tttcgaaagg gcagattgtg tggacaggta
      481 atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc
      541 tgctagttga acgcttccat cttcaatgtt gtgtctaatt ttgaagttaa ctttgattcc
      601 attcttttgt ttgtctgcca tgatgtatac attgtgtgag ttatagttgt attccaattt
      661 gtgtccaaga atgtttccat cttctttaaa atcaatacct tttaactcga ttctattaac
      721 aagggtatca ccttcaaact tgacttcagc acgtgtcttg tagttcccgt catctttgaa
      781 aaatatagtt ctttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgccg
      841 tttcatatga tctgggtatc ttgaaaagca ttgaacacca tacgtaaaag tagtgacaag
      901 tgttggccat g
//
