LOCUS       sequence-161460-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with 
            kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas27
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gccaagctta attaagtcaa gggatggaga aagcaaaatg aataaactgt ttacagcagc
       61 ccttagtggt ctatcattta tcgttgttca acaggcaaaa agtgaacctg tatttattga
      121 ttttgattac cagtatcggt atggttatga atataattac gactatgatg actataccta
      181 ttactaccac gccaatgtct acacctatag cgatgattcg gttgtattcc cagtcaaagt
      241 actcgttaat gctgcatgtc aacaaccaca ataccccatt gtattaggct ctgaagcaga
      301 tataagcaac ggtacagatg tcttgatcga agaatctaat gtcattattg ataatgattt
      361 cagtacaaat tgctctgctt ttacagaaaa aaatgtcctg ctactctggg aagaaaacga
      421 gataaaatag gcgtcccttc tggtttttct ttctgtaaag ctgtactgtt taaaaataga
      481 tcttaaatta tagaagcact ttgactttat tttcggatcc accttttcgg ggaaatgtgc
      541 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt
      601 aattcttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
      661 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
      721 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
      781 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
      841 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca
      901 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
      961 gattgcgcct gagcgagacg aaatacgcga tcg
//
