LOCUS       sequence-161462-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Calreticulin mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Wijeyesakere SJ, Bedi SK, Huynh D, Raghavan M
  TITLE     The C-Terminal Acidic Region of Calreticulin Mediates 
            Phosphatidylserine Binding and Apoptotic Cell Phagocytosis.
  JOURNAL   J Immunol. 2016 May 1;196(9):3896-909. doi: 
            10.4049/jimmunol.1502122. Epub 2016 Apr 1.
  PUBMED    27036911
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1502122"; journalName: "J Immunol"; date: 
            "2016-05-1- 1"; volume: "196"; issue: "9"; pages: "3896-909"
COMMENT     Sequence Label: pGB1-mCRT(C-domain)
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..252
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLALNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             259..279
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             532..549
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(602..620)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      616..663
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(787..806)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgcag tacaagcttg ctctgaacgg taaaaccctg
      121 aaaggtgaaa ccaccaccga agctgttgac gctgctaccg cggaaaaagt tttcaaacag
      181 tacgctaacg acaacggtgt tgacggtgaa tggacctacg acgacgctac caaaaccttc
      241 acggtaaccg aaggtaccga gaacctgtac ttccaatcca atgctatgaa ggacaagcag
      301 gatgaggagc agaggcttaa ggaagaagaa gaggacaaga agcgtaaaga ggaagaagaa
      361 gctgaggata aagaggatga tgatgacaga gatgaagatg aggatgaaga agatgagaag
      421 gaggaagatg aggaagaatc ccctggccaa gccaaggatg agctgtagca ttggaagtgg
      481 ataacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac
      541 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      601 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      661 ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat
      721 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      781 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      841 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      901 ccaaaaaact tgattagggt gatggttcac attaacgcct tacaatttta ggtggc
//
