LOCUS       sequence-161463-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET probe for measuring tension on Talin molecule.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Kumar A, Ouyang M, Van den Dries K, McGhee EJ, Tanaka K, Anderson 
            MD, Groisman A, Goult BT, Anderson KI, Schwartz MA
  TITLE     Talin tension sensor reveals novel features of focal adhesion force 
            transmission and mechanosensitivity.
  JOURNAL   J Cell Biol. 2016 May 9;213(3):371-83. doi: 10.1083/jcb.201510012.
  PUBMED    27161398
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2016-05-9- 9"; volume: "213"; 
            issue: "3"; pages: "371-83"
COMMENT     Sequence Label: Talin-TS
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(252..254,255..257,258..770)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(297..318)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(558..577)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttactctgga ctttggagac tatcaggatg gctactactc agtacagacg acagaaggcg
       61 agcagatcgc acagctcatt gctggctaca tagatatcat ccttaagaag aaaaaaagca
      121 aggaccattt tgggctggag ggagatgaag agtctactat gttggaggac tcagtgtctc
      181 ccaaaaagtc aacagtcctt cagcagcagt acaaccgagt ggggaaagtg gagcacggct
      241 ctgtggtcga gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      301 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      361 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      421 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
      481 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      541 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      601 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
      661 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
      721 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
      781 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      841 ccgacaacca ctacctgagc acc
//
