LOCUS       sequence-161469-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  untargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pair.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Yoo TY, Choi JM, Conway W, Yu CH, Pappu RV, Needleman DJ
  TITLE     Measuring NDC80 binding reveals the molecular basis of 
            tension-dependent kinetochore-microtubule attachments.
  JOURNAL   Elife. 2018 Jul 25;7. pii: 36392. doi: 10.7554/eLife.36392.
  PUBMED    30044223
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-25"; volume: "7. pii: 36392. doi"; pages: " 
            10.7554/eLife.36392"
COMMENT     Sequence Label: pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(13..723,724..726))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
     primer_bind     404..423
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     663..684
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cgcttaagct cgcttgtaca gctcgttcat gccttcagtg atcccggcgg cggtcaggaa
       61 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgaacaggg cggactggta
      121 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      181 gtagtggtcg gcgagctgca cgccgccgtc ctcgatgttg tggcggatct tgaagttggc
      241 cttgatgccg ttcttctgct tgtcggcggt gatatagacg ttgtggctgt tgtagttgta
      301 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      361 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      421 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
      481 gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac tgcaggccgt agcccagggt
      541 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagagca gcttcagggt
      601 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
      661 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
      721 gctcactcct ccggaaccac cgatggccac ggtgcggcgg ttcttcacga tcttgttcac
      781 cttgccgccg gatcctccta actttttcac caaatctt
//
