LOCUS       sequence-161475-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of exogenous SL1(G20A) in C. elegans muscle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Ma X, Zhan G, Sleumer MC, Chen S, Liu W, Zhang MQ, Liu X
  TITLE     Analysis of C. elegans muscle transcriptome using 
            trans-splicing-based RNA tagging (SRT).
  JOURNAL   Nucleic Acids Res. 2016 Aug 23. pii: gkw734.
  PUBMED    27557708
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 23. pii: gkw734."
COMMENT     Sequence Label: Pmyo-3::tag:SL1(G20A)
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..29
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13..29
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     48..68
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        50..68
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     93..109
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     94..110
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ataagttctt gacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
       61 actaaaggga acaaaagctg ggtaccgggc cctcgaggtc gacggtatcg ataagcttga
      121 tatcgaattc gttccccaat aatatcatca ccttcccaca tctccacata cggacagcca
      181 tgctccctca tctccacata ttttcgaatg gaccgttctc caacatggat gaaccgattt
      241 tcgaatttca ttaccctgtt atccctatta gtatttttgt ctccccgaga ggagacgttc
      301 caaaatttat agctaacgcc aaatttcttt gggtcagttt caatgtttac cttaaacttg
      361 ggtaattaaa ccagatcgga agagcgtcgt gtaattgaat tgtattgagc cttcggagcg
      421 agttgtgggg gagcaacaga atctatacaa cacgcacaca catcatcgtt gaagataaag
      481 aagaagaaaa cggaatcccg ggagctatcc cgccctctct gcactctctc tctaatattc
      541 tattcttgct cttctatttt cacttcggga gcccaaccaa cacctaccct ccttcgtttt
      601 ccgatgaact tgtgtttaga gacatagaga tatcagagaa gggacacaaa ttttgttttt
      661 aacaaatcgc cgagaaattt gaagaaaggt gatgaaaaac tgggtgctat tgttagccag
      721 tggggaatgt aactgggaat accctagggt tatgaaactt ttcagttctt acacaggtaa
      781 agctggattt tccaaatcat caatcggttc ctctgcagtc tcgattaaaa tatttgatat
      841 tttcgtcagg gattttgaag acaa
//
