LOCUS       sequence-161479-        6242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of exogenous SL1(G20A) in C. elegans muscle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Ma X, Zhan G, Sleumer MC, Chen S, Liu W, Zhang MQ, Liu X
  TITLE     Analysis of C. elegans muscle transcriptome using 
            trans-splicing-based RNA tagging (SRT).
  JOURNAL   Nucleic Acids Res. 2016 Aug 23. pii: gkw734.
  PUBMED    27557708
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 23. pii: gkw734."
COMMENT     Sequence Label: Pmyo-3::tag:SL1(G20A)
FEATURES             Location/Qualifiers
     source          1..6242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2529..2545)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2530..2546)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2571..2591)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2571..2589)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2610..2626)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2675..2694)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2676..2694)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2701..2718)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2701..2717)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2710..2732)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2858..3286
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2945..2964)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3155..3176
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             3630..4424
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3684..3703)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4294..4313
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(4442..4510,4511..5302)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4660..4679)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5473..6061
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5962..5981
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6215..6232
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctgc
      241 ccttcgcgac cggtcggcta taataagttc ttgaataaaa taattttccc gacaaaacat
      301 gagtatttct ttcgaaaata aaagtgcagg ctaattagag attattctgt aattaactgc
      361 ataatttgtc acgtgccata gttttacatt ccactacgtc atagttctta aaatactaat
      421 ctcctgaaaa tagaagtagg tgaagaaagt ttaattatca gttctaaaat gacaattgat
      481 ctttggaata tgttctgaaa ctaccgatca ttgaacagat gctatttgaa tgatatagaa
      541 ttgtatattt gcaatttctg aaacgcgttc ttaaaggcac acagattaat tcaaaagggt
      601 ctggccgcaa aaaggtttat ggtggccgat tttgagtttt gtgtgtgatt gctttttcac
      661 aatcagtgtt ttcaggatta tgtgatgaac tagatcttca agtttcgtta catttcatat
      721 gttttcggaa ctcacgaagt acatattggg tattgtgctc aaaaaattca gcaatcagct
      781 tcgctccgct gactttagaa cccaaaaaaa tagtatggcc aaactgactg tgttacgatc
      841 atttcaattt ttcaatacat atttaagatt tctaagagta agaaggtcaa aaactgttct
      901 ggaatacata tatatttttc aggttacaat tagtcaaaaa gtgcactgaa atatacgttt
      961 taatttcacg aataacccaa ttagttcaat gtatttttgg tcaaccaacg ttaaagtttg
     1021 gcttccaacc aattatcatt tctgatcaac cacaatgttt tttctttatc tgcaagttaa
     1081 ttttttattt ttatccagat gtttggcata tttttcaatt cttcactagc gcccacttct
     1141 tgcacttccg gcgccctgaa tctaatgcat ctgttgcaag aattgaaaga ccaatcaaca
     1201 cattgttttc ttcacgagat actgaagaaa atgaataaaa acagagaaaa agagccatgt
     1261 gattagtgac aactgttgct aacagatacc atagcttgga cttggtacgt gatggcaacg
     1321 tatgggtcaa caaaaatgat tgcagagggg gtgcaaaaca gtcaagtcga gaaaatatga
     1381 aaaacagaaa acaaagaaca gaaaaatggg tttgagagtc agtataattt ataaaagaaa
     1441 aattgtacat agaaattaac catttttgta gaagaagtta tttttcaagc atcgttaaaa
     1501 attattcaaa gcaccttatt tcatatttaa ttttaaacat ggttaaatga acaacacggt
     1561 gcgcaatcag gaaaacttga aatctgaaac tgttgttgtg atcttcttcg caactgttca
     1621 gatagcacta gtgtaatgtt aagagtgcgc gaatataatg gaatataatg gatcacacct
     1681 cctgccatca ggtaaacgtc tctgttatca catatttcca actattaaat ttttaccttt
     1741 tacagtttta catttttttg aaaaaagtaa ctttttgtct tcaaaatccc tgacgaaaat
     1801 atcaaatatt ttaatcgaga ctgcagagga accgattgat gatttggaaa atccagcttt
     1861 acctgtgtaa gaactgaaaa gtttcataac cctagggtat tcccagttac attccccact
     1921 ggctaacaat agcacccagt ttttcatcac ctttcttcaa atttctcggc gatttgttaa
     1981 aaacaaaatt tgtgtccctt ctctgatatc tctatgtctc taaacacaag ttcatcggaa
     2041 aacgaaggag ggtaggtgtt ggttgggctc ccgaagtgaa aatagaagag caagaataga
     2101 atattagaga gagagtgcag agagggcggg atagctcccg ggattccgtt ttcttcttct
     2161 ttatcttcaa cgatgatgtg tgtgcgtgtt gtatagattc tgttgctccc ccacaactcg
     2221 ctccgaaggc tcaatacaat tcaattacac gacgctcttc cgatctggtt taattaccca
     2281 agtttaaggt aaacattgaa actgacccaa agaaatttgg cgttagctat aaattttgga
     2341 acgtctcctc tcggggagac aaaaatacta atagggataa cagggtaatg aaattcgaaa
     2401 atcggttcat ccatgttgga gaacggtcca ttcgaaaata tgtggagatg agggagcatg
     2461 gctgtccgta tgtggagatg tgggaaggtg atgatattat tggggaacga attcgatatc
     2521 aagcttatcg ataccgtcga cctcgagggc ccggtaccca gcttttgttc cctttagtga
     2581 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtcaa gaacttatta
     2641 tagccgaccg gtcgcgaagg gcagcttcaa ttcgccctat agtgagtcgt attacaattc
     2701 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     2761 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     2821 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
     2881 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2941 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3001 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3061 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3121 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3181 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     3241 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaattcag
     3301 ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct
     3361 gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga
     3421 gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga
     3481 cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca
     3541 aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagatctg
     3601 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     3661 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     3721 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     3781 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     3841 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     3901 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atcccacctt gctcctgccg
     3961 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     4021 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4081 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4141 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc cacggcgatg
     4201 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     4261 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     4321 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     4381 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattgaa aaaggaagag
     4441 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4501 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4561 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4621 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4681 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4741 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4801 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4861 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4921 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4981 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5041 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5101 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5161 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5221 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5281 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5341 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5401 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5461 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5521 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5581 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5641 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5701 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5761 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5821 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5881 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5941 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6001 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6061 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6121 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6181 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6241 ag
//
