LOCUS       sequence-161497-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses full length sfCherry2 and TagBFP fusion in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pSFFV_sfCherry2_TagBFP
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     549..567
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttcctggtgc aggattcgaa gcgccgccgg tgctgtgtct ggcctcggct ctctcgtact
       61 gctccacgat ggtgtagtcc tcgttgtggc tggtgatgtc cagcttgatg tccacgttgt
      121 aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt ggtcttcacc tcggcgtcgt
      181 agtggccgcc gtccttcagc ttcagtctct ggttgatctc gcccttcagg gcgccgtcct
      241 cggggtacat tctctcggtg ctggcctccc agcccatggt cttcttctgc atcacggggc
      301 cgtcgctggg gaagttgatg cccagcagct tcaccttgta gatgaactgg ccgtcctgca
      361 ggctgctgtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc actctctccc
      421 aggtgaagcc ctcggggaag ctcagcttca ggtagtcggg gatgtcggcg gggtgcttca
      481 cgtaggcctt gctgccgtac atgaactggg ggctcaggat gtcccaggcg aagggcaggg
      541 ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg tggccctcgc
      601 cctcgccctc gatctcgaac tcgtggccgt tcacgctgcc ctccatgtgc accttgaatc
      661 tcatgaactc cttgatgatg gccatgttgt cctcctccat ggatcccggg cccgcggtac
      721 cgcaagcttg atatcctgca gacgcgtgag aattctcgag agctccagat cccccccggg
      781 cgactcagtc tgtcggagga ctggcgcgcc gagtgagggg ttgtgagctc ttttatagag
      841 ctcggga
//
