LOCUS       sequence-161501-       11635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid that expresses VSVG protein under a 
            doxycycline-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11635)
  TITLE     SPReAD plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV puro Tet VSVG
FEATURES             Location/Qualifiers
     source          1..11635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    6..230
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(246..845)
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(329..349)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     826..845
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(855..1184)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      869..1004
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    join(1216..1234,1235..1257,1258..1276,1277..1299,1300..1318,
                     1319..1341,1342..1360,1361..1383,1384..1402,1403..1425,
                     1426..1444,1445..1467,1468..1486)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        1519..1556
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1553..1577
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1673..1790
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             join(1849..1896,1897..3384)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     misc_feature    3445..4033
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3498..3518)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3916..3927)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4177..4210)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4230..4410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4435..4453)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4436..4453)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4743..4762)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4976..4995)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5095..5117
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5155..5173)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5241..5345
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5346..5414,5415..6206)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5564..5583)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6377..6965
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6866..6885
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7119..7136
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        7211..7540
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7391..7526
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8674..8739
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8869..8889
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9314..9448
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9351..9370)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9405..9424
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9617..10250
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10297..10422
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10915..11148
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11333..11377
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgatgccata gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc
       61 cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga
      121 tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga
      181 aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag gtcgacggta
      241 tcgattcagg caccgggctt gcgggtcatg caccaggtcg cgcggtcctt cgggcactcg
      301 acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag
      361 gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg gagcacgacg
      421 gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc caggaaccac
      481 gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg cgcggccagc
      541 cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg
      601 acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg
      661 ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac ccgctcgatg
      721 tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg
      781 gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg
      841 gtcatggtaa gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
      901 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
      961 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     1021 gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     1081 cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg
     1141 cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca gtcagcttct
     1201 ggtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     1261 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     1321 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     1381 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     1441 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     1501 gctcggtacc cgggtcgagt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
     1561 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     1621 accgggaccg atccagcctc cgcggaattt aatctcgacg gtatcggtta acttttaaaa
     1681 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     1741 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg
     1801 agactagcct cgaggtttaa acttaagctt ggtaccgagc tcggatccat gaagtgcctt
     1861 ttgtacttag cctttttatt cattggggtg aattgcaagt tcaccatagt ttttccacac
     1921 aaccaaaaag gaaactggaa aaatgttcct tctaattacc attattgccc gtcaagctca
     1981 gatttaaatt ggcataatga cttaataggc acagccttac aagtcaaaat gcccaagagt
     2041 cacaaggcta ttcaagcaga cggttggatg tgtcatgctt ccaaatgggt cactacttgt
     2101 gatttccgct ggtatggacc gaagtatata acacattcca tccgatcctt cactccatct
     2161 gtagaacaat gcaaggaaag cattgaacaa acgaaacaag gaacttggct gaatccaggc
     2221 ttccctcctc aaagttgtgg atatgcaact gtgacggatg ccgaagcagt gattgtccag
     2281 gtgactcctc accatgtgct ggttgatgaa tacacaggag aatgggttga ttcacagttc
     2341 atcaacggaa aatgcagcaa ttacatatgc cccactgtcc ataactctac aacctggcat
     2401 tctgactata aggtcaaagg gctatgtgat tctaacctca tttccatgga catcaccttc
     2461 ttctcagagg acggagagct atcatccctg ggaaaggagg gcacagggtt cagaagtaac
     2521 tactttgctt atgaaactgg aggcaaggcc tgcaaaatgc aatactgcaa gcattgggga
     2581 gtcagactcc catcaggtgt ctggttcgag atggctgata aggatctctt tgctgcagcc
     2641 agattccctg aatgcccaga agggtcaagt atctctgctc catctcagac ctcagtggat
     2701 gtaagtctaa ttcaggacgt tgagaggatc ttggattatt ccctctgcca agaaacctgg
     2761 agcaaaatca gagcgggtct tccaatctct ccagtggatc tcagctatct tgctcctaaa
     2821 aacccaggaa ccggtcctgc tttcaccata atcaatggta ccctaaaata ctttgagacc
     2881 agatacatca gagtcgatat tgctgctcca atcctctcaa gaatggtcgg aatgatcagt
     2941 ggaactacca cagaaaggga actgtgggat gactgggcac catatgaaga cgtggaaatt
     3001 ggacccaatg gagttctgag gaccagttca ggatataagt ttcctttata catgattgga
     3061 catggtatgt tggactccga tcttcatctt agctcaaagg ctcaggtgtt cgaacatcct
     3121 cacattcaag acgctgcttc gcaacttcct gatgatgaga gtttattttt tggtgatact
     3181 gggctatcca aaaatccaat cgagcttgta gaaggttggt tcagtagttg gaaaagctct
     3241 attgcctctt ttttctttat catagggtta atcattggac tattcttggt tctccgagtt
     3301 ggtatccatc tttgcattaa attaaagcac accaagaaaa gacagattta tacagacata
     3361 gagatgaacc gacttggaaa gtgataagaa ttctgcagat atccagcaca gtggcggccg
     3421 ctcgagtcta gacatatggc tgataatcaa cctctggatt acaaaatttg tgaaagattg
     3481 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3541 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3601 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3661 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3721 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3781 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     3841 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3901 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3961 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4021 gccgcctccc cgcatcggta cgtatggcca ggtaccttta agaccaatga cttacaaggc
     4081 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     4141 ccaacgaaga caagatggga tcaattcacc atgggaataa cttcgtatag catacattat
     4201 acgaagttat gctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     4261 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     4321 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     4381 ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata
     4441 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
     4501 taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc
     4561 atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg
     4621 taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca
     4681 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     4741 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     4801 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     4861 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     4921 ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa
     4981 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5041 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5101 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5161 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5221 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5281 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5341 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5401 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5461 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5521 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5581 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5641 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5701 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5761 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5821 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5881 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5941 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6001 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6061 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6121 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6181 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6241 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6301 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6361 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6421 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6481 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     6541 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6601 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6661 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6721 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     6781 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6841 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6901 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6961 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7021 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7081 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7141 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7201 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     7261 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     7321 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     7381 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7441 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7501 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
     7561 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
     7621 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
     7681 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
     7741 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
     7801 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
     7861 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     7921 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
     7981 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
     8041 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     8101 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
     8161 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
     8221 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
     8281 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     8341 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     8401 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     8461 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
     8521 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
     8581 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     8641 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     8701 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
     8761 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     8821 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
     8881 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
     8941 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
     9001 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
     9061 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
     9121 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
     9181 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
     9241 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     9301 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     9361 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     9421 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
     9481 caaacttccc accccatacc ctattaccac tgccaattac ctagtggttt catttactct
     9541 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     9601 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     9661 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     9721 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     9781 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     9841 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     9901 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     9961 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
    10021 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
    10081 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    10141 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    10201 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
    10261 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
    10321 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
    10381 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
    10441 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
    10501 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
    10561 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
    10621 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    10681 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    10741 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
    10801 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
    10861 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
    10921 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
    10981 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
    11041 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
    11101 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
    11161 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
    11221 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
    11281 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
    11341 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
    11401 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
    11461 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
    11521 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
    11581 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtat
//
