LOCUS       sequence-161502-        5139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CTNNB1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_CTNNB1_p.F777S
FEATURES             Location/Qualifiers
     source          1..5139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     100..117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(276..303)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(395..481)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     530..552
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     544..561
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     545..561
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    577..676
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             679..3021
                     /codon_start=1
                     /gene="Homo sapiens CTNNB1"
                     /product="human cadherin-associated protein that also plays
                     a role in the canonical Wnt signaling pathway"
                     /label=beta-catenin
                     /note="also known as catenin beta-1"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWSDTDL"
     protein_bind    complement(3026..3125)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3142..3161)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3143..3161)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3166..3182)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3166..3182)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3613..4404
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4497..5085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4986..5005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
       61 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      121 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      181 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
      241 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
      301 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
      361 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
      421 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
      481 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
      541 cgttgtaaaa cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg
      601 atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg
      661 tacaaaaaag ttggcaccat ggctactcaa gctgatttga tggagttgga catggccatg
      721 gaaccagaca gaaaagcggc tgttagtcac tggcagcaac agtcttacct ggactctgga
      781 atccattctg gtgccactac cacagctcct tctctgagtg gtaaaggcaa tcctgaggaa
      841 gaggatgtgg atacctccca agtcctgtat gagtgggaac agggattttc tcagtccttc
      901 actcaagaac aagtagctga tattgatgga cagtatgcaa tgactcgagc tcagagggta
      961 cgagctgcta tgttccctga gacattagat gagggcatgc agatcccatc tacacagttt
     1021 gatgctgctc atcccactaa tgtccagcgt ttggctgaac catcacagat gctgaaacat
     1081 gcagttgtaa acttgattaa ctatcaagat gatgcagaac ttgccacacg tgcaatccct
     1141 gaactgacaa aactgctaaa tgacgaggac caggtggtgg ttaataaggc tgcagttatg
     1201 gtccatcagc tttctaaaaa ggaagcttcc agacacgcta tcatgcgttc tcctcagatg
     1261 gtgtctgcta ttgtacgtac catgcagaat acaaatgatg tagaaacagc tcgttgtacc
     1321 gctgggacct tgcataacct ttcccatcat cgtgagggct tactggccat ctttaagtct
     1381 ggaggcattc ctgccctggt gaaaatgctt ggttcaccag tggattctgt gttgttttat
     1441 gccattacaa ctctccacaa ccttttatta catcaagaag gagctaaaat ggcagtgcgt
     1501 ttagctggtg ggctgcagaa aatggttgcc ttgctcaaca aaacaaatgt taaattcttg
     1561 gctattacga cagactgcct tcaaatttta gcttatggca accaagaaag caagctcatc
     1621 atactggcta gtggtggacc ccaagcttta gtaaatataa tgaggaccta tacttacgaa
     1681 aaactactgt ggaccacaag cagagtgctg aaggtgctat ctgtctgctc tagtaataag
     1741 ccggctattg tagaagctgg tggaatgcaa gctttaggac ttcacctgac agatccaagt
     1801 caacgtcttg ttcagaactg tctttggact ctcaggaatc tttcagatgc tgcaactaaa
     1861 caggaaggga tggaaggtct ccttgggact cttgttcagc ttctgggttc agatgatata
     1921 aatgtggtca cctgtgcagc tggaattctt tctaacctca cttgcaataa ttataagaac
     1981 aagatgatgg tctgccaagt gggtggtata gaggctcttg tgcgtactgt ccttcgggct
     2041 ggtgacaggg aagacatcac tgagcctgcc atctgtgctc ttcgtcatct gaccagccga
     2101 caccaagaag cagagatggc ccagaatgca gttcgccttc actatggact accagttgtg
     2161 gttaagctct tacacccacc atcccactgg cctctgataa aggctactgt tggattgatt
     2221 cgaaatcttg ccctttgtcc cgcaaatcat gcacctttgc gtgagcaggg tgccattcca
     2281 cgactagttc agttgcttgt tcgtgcacat caggataccc agcgccgtac gtccatgggt
     2341 gggacacagc agcaatttgt ggagggggtc cgcatggaag aaatagttga aggttgtacc
     2401 ggagcccttc acatcctagc tcgggatgtt cacaaccgaa ttgttatcag aggactaaat
     2461 accattccat tgtttgtgca gctgctttat tctcccattg aaaacatcca aagagtagct
     2521 gcaggggtcc tctgtgaact tgctcaggac aaggaagctg cagaagctat tgaagctgag
     2581 ggagccacag ctcctctgac agagttactt cactctagga atgaaggtgt ggcgacatat
     2641 gcagctgctg ttttgttccg aatgtctgag gacaagccac aagattacaa gaaacggctt
     2701 tcagttgagc tgaccagctc tctcttcaga acagagccaa tggcttggaa tgagactgct
     2761 gatcttggac ttgatattgg tgcccaggga gaaccccttg gatatcgcca ggatgatcct
     2821 agctatcgtt cttttcactc tggtggatat ggccaggatg ccttgggtat ggaccccatg
     2881 atggaacatg agatgggtgg ccaccaccct ggtgctgact atccagttga tgggctgcca
     2941 gatctggggc atgcccagga cctcatggat gggctgcctc caggtgacag caatcagctg
     3001 gcctggtctg atactgacct gtgattgcca actttcttgt acaaagttgg cattataaga
     3061 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     3121 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     3181 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     3241 tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag
     3301 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
     3361 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     3421 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     3481 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     3541 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     3601 aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     3661 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     3721 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     3781 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     3841 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     3901 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     3961 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     4021 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     4081 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     4141 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     4201 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     4261 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     4321 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     4381 atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg
     4441 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4621 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     4681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5101 gcctttttac ggttcctggc cttttgctgg ccttttgct
//
