LOCUS       sequence-161523-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Corynebacterium diphtheriae Cas9 (CdiCas9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Ma E, Harrington LB, O'Connell MR, Zhou K, Doudna JA
  TITLE     Single-Stranded DNA Cleavage by Divergent CRISPR-Cas9 Enzymes.
  JOURNAL   Mol Cell. 2015 Nov 5;60(3):398-407. doi: 
            10.1016/j.molcel.2015.10.030.
  PUBMED    26545076
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10"; journalName: "Mol Cell"; date: 
            "2015-11-5- 5"; volume: "60"; issue: "3"; pages: "398-407"
COMMENT     Sequence Label: p2CT-Cdi
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             41..63
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             83..112
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 attagcggcc gccctgcagg actcgagttc tagaaataat tttgtttaac tttaagaagg
       61 agatatacat atgaaatctt ctcaccatca ccatcaccat caccatcacc atggttcttc
      121 tatgaaaatc gaagaaggta aactggtaat ctggattaac ggcgataaag gctataacgg
      181 tctcgctgaa gtcggtaaga aattcgagaa agataccgga attaaagtca ccgttgagca
      241 tccggataaa ctggaagaga aattcccaca ggttgcggca actggcgatg gccctgacat
      301 tatcttctgg gcacacgacc gctttggtgg ctacgctcaa tctggcctgt tggctgaaat
      361 caccccggac aaagcgttcc aggacaagct gtatccgttt acctgggatg ccgtacgtta
      421 caacggcaag ctgattgctt acccgatcgc tgttgaagcg ttatcgctga tttataacaa
      481 agatctgctg ccgaacccgc caaaaacctg ggaagagatc ccggcgctgg ataaagaact
      541 gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa gaaccgtact tcacctggcc
      601 gctgattgct gctgacgggg gttatgcgtt caagtatgaa aacggcaagt acgacattaa
      661 agacgtgggc gtggataacg ctggcgcgaa agcgggtctg accttcctgg ttgacctgat
      721 taaaaacaaa cacatgaatg cagacaccga ttactccatc gcagaagctg cctttaataa
      781 aggcgaaaca gcgatgacca tcaacgggcc cgtgggcatg gtccaacatc gacaccagca
      841 aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg
      901 ttggcgtgct gagcgcaggt attaacgccg c
//
