LOCUS       sequence-161530-         349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP from the CBA promoter. Includes a Csy4 target hairpin 
            in the 3'UTR. Cloned into an Adeno-Associated Virus backbone for 
            packaging into AAV..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Borchardt EK, Vandoros LA, Huang M, Lackey PE, Marzluff WF, Asokan A
  TITLE     Controlling mRNA stability and translation with the CRISPR 
            endoribonuclease Csy4.
  JOURNAL   RNA. 2015 Nov;21(11):1921-30. doi: 10.1261/rna.051227.115. Epub 2015
            Sep 9.
  PUBMED    26354771
REFERENCE   2  (bases 1 to 349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.051227.115"; journalName: "RNA"; date: "2015-11"; 
            volume: "21"; issue: "11"; pages: "1921-30"
COMMENT     Sequence Label: TR-GFP-3'UTR-HP
FEATURES             Location/Qualifiers
     source          1..349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    53..72
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     polyA_signal    81..202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(92..111)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     146..165
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 catggacgag ctgtacaagt aactcgagcg gccgcttcga gcagacatga tagttcactg
       61 ccgtataggc agctaagaaa taagatacat tgatgagttt ggacaaacca caactagaat
      121 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      181 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
      241 gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga
      301 taaggatctg aggaacccct agtgatggag ttggccactc cctctctgc
//
