LOCUS       sequence-161533-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre dependent expression of GCaMP6f Ca sensor and physically 
            separate nuclear localized dTomato fluorophore .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  TITLE     Ting GCaMP6 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(90..111)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(351..370)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatggtg agcaagggcg
       61 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      121 acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctacggcaag ctgaccctga
      181 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      241 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      301 agtccgccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag gacgacggca
      361 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      421 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacc
      481 tgccggacca actgactgaa gagcagatcg cagaatttaa agaggaattc tccctatttg
      541 acaaggacgg ggatgggaca ataacaacca aggagctggg gacggtgatg cggtctctgg
      601 ggcagaaccc cacagaagca gagctgcagg acatgatcaa tgaagtagat gccgacggtg
      661 acggcacaat cgacttccct gagttcctga caatgatggc aagaaaaatg aaatacaggg
      721 acacggaaga agaaattaga gaagcgttcg gtgtgtttga taaggatggc aatggctaca
      781 tcagtgcagc agagcttcgc cacgtgatga caaaccttgg agagaagtta acagatgaag
      841 aggttgatga aa
//
