LOCUS       sequence-161537-         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Csy4 from the CBA promoter. Cloned into an 
            Adeno-Associated Virus backbone for packaging into AAV..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Borchardt EK, Vandoros LA, Huang M, Lackey PE, Marzluff WF, Asokan A
  TITLE     Controlling mRNA stability and translation with the CRISPR 
            endoribonuclease Csy4.
  JOURNAL   RNA. 2015 Nov;21(11):1921-30. doi: 10.1261/rna.051227.115. Epub 2015
            Sep 9.
  PUBMED    26354771
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.051227.115"; journalName: "RNA"; date: "2015-11"; 
            volume: "21"; issue: "11"; pages: "1921-30"
COMMENT     Sequence Label: TR-Csy4
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..569)
                     /codon_start=1
                     /product="CRISPR-associated endoribonuclease responsible 
                     for pre-crRNA processing in Pseudomonas aeruginosa 
                     (Haurwitz et al., 2010)"
                     /label=Csy4
                     /translation="MDHYLDIRLRPDPEFPPAQLMSVLFGKLHQALVAQGGDRIGVSFP
                     DLDESRSRLGERLRIHASADDLRALLARPWLEGLRDHLQFGEPAVVPHPTPYRQVSRVQ
                     AKSNPERLRRRLMRRHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLFIRHGP
                     LQVTAEEGGFTCYGLSKGGFVPWF"
     primer_bind     complement(582..604)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gccgctcaga accagggaac gaaacctcct ttgctcaacc cgtaacaggt gaatcctcct
       61 tcctctgccg tcacctgcaa cggcccgtgg cggatgaaga gacggaagtg ctgtccggtg
      121 ctctggctgc gtagcgtgac gaagggcagg tccaaggctc tcgcgaccgt atcgggaatg
      181 cgtttccgag cctcctcctc actcagatcg tgccggcgca tgagccgccg ccgcaggcgt
      241 tccggattgc ttttcgcctg aacccgactg acctgacggt acggtgtggg gtgaggcacg
      301 actgccggtt ctccgaattg cagatggtcc cgcaaccctt ccagccaggg ccgggcgagc
      361 agggcacgaa ggtcgtccgc cgaggcatga atgcgcaggc gctcgcccag ccgggagcgg
      421 ctttcgtcga ggtcggggaa gctcacgccg atcctgtccc cgccctgtgc caccagggcc
      481 tggtggagct tgccgaagag cacgctcatg agttgcgccg ggggaaattc cgggtccggt
      541 cgcaagcgaa tgtcgaggta gtggtccatt ctagagccgc cggtcacacg ccagaagccg
      601 aaccccgccc tg
//
