LOCUS       sequence-161541-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Csy4 with R115A and R119A mutations from the CBA promoter.
            Cloned into an Adeno-Associated Virus backbone for packaging into 
            AAV..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Borchardt EK, Vandoros LA, Huang M, Lackey PE, Marzluff WF, Asokan A
  TITLE     Controlling mRNA stability and translation with the CRISPR 
            endoribonuclease Csy4.
  JOURNAL   RNA. 2015 Nov;21(11):1921-30. doi: 10.1261/rna.051227.115. Epub 2015
            Sep 9.
  PUBMED    26354771
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.051227.115"; journalName: "RNA"; date: "2015-11"; 
            volume: "21"; issue: "11"; pages: "1921-30"
COMMENT     Sequence Label: TR-Csy4-R115A/R119A
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    579..700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(590..609)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     644..663
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tacctcgaca ttcgcttgcg accggacccg gaatttcccc cggcgcaact catgagcgtg
       61 ctcttcggca agctccacca ggccctggtg gcacagggcg gggacaggat cggcgtgagc
      121 ttccccgacc tcgacgaaag ccgctcccgg ctgggcgagc gcctgcgcat tcatgcctcg
      181 gcggacgacc ttcgtgccct gctcgcccgg ccctggctgg aagggttgcg ggaccatctg
      241 caattcggag aaccggcagt cgtgcctcac cccacaccgt accgtcaggt cagtcgggtt
      301 caggcgaaaa gcaatccgga acgcctgcgg cgggccctca tgcgcgccca cgatctgagt
      361 gaggaggagg ctcggaaacg cattcccgat acggtcgcga gagccttgga cctgcccttc
      421 gtcacgctac gcagccagag caccggacag cacttccgtc tcttcatccg ccacgggccg
      481 ttgcaggtga cggcagagga aggaggattc acctgttacg ggttgagcaa aggaggtttc
      541 gttccctggt tctgagcggc cgcttcgagc agacatgata agatacattg atgagtttgg
      601 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      661 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
      721 ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta
      781 caaatgtggt aaaatcgata aggatctgag gaacccctag tgatggagtt gg
//
