LOCUS       sequence-161542-         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Csy4 with R115A and R119A mutations from the CBA promoter.
            Cloned into an Adeno-Associated Virus backbone for packaging into 
            AAV..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Borchardt EK, Vandoros LA, Huang M, Lackey PE, Marzluff WF, Asokan A
  TITLE     Controlling mRNA stability and translation with the CRISPR 
            endoribonuclease Csy4.
  JOURNAL   RNA. 2015 Nov;21(11):1921-30. doi: 10.1261/rna.051227.115. Epub 2015
            Sep 9.
  PUBMED    26354771
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.051227.115"; journalName: "RNA"; date: "2015-11"; 
            volume: "21"; issue: "11"; pages: "1921-30"
COMMENT     Sequence Label: TR-Csy4-R115A/R119A
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..582)
                     /codon_start=1
                     /product="CRISPR-associated endoribonuclease responsible 
                     for pre-crRNA processing in Pseudomonas aeruginosa 
                     (Haurwitz et al., 2010)"
                     /label=Csy4
                     /translation="MDHYLDIRLRPDPEFPPAQLMSVLFGKLHQALVAQGGDRIGVSFP
                     DLDESRSRLGERLRIHASADDLRALLARPWLEGLRDHLQFGEPAVVPHPTPYRQVSRVQ
                     AKSNPERLRRALMRAHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLFIRHGP
                     LQVTAEEGGFTCYGLSKGGFVPWF"
     primer_bind     complement(595..617)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 tctgctcgaa gcggccgctc agaaccaggg aacgaaacct cctttgctca acccgtaaca
       61 ggtgaatcct ccttcctctg ccgtcacctg caacggcccg tggcggatga agagacggaa
      121 gtgctgtccg gtgctctggc tgcgtagcgt gacgaagggc aggtccaagg ctctcgcgac
      181 cgtatcggga atgcgtttcc gagcctcctc ctcactcaga tcgtgggcgc gcatgagggc
      241 ccgccgcagg cgttccggat tgcttttcgc ctgaacccga ctgacctgac ggtacggtgt
      301 ggggtgaggc acgactgccg gttctccgaa ttgcagatgg tcccgcaacc cttccagcca
      361 gggccgggcg agcagggcac gaaggtcgtc cgccgaggca tgaatgcgca ggcgctcgcc
      421 cagccgggag cggctttcgt cgaggtcggg gaagctcacg ccgatcctgt ccccgccctg
      481 tgccaccagg gcctggtgga gcttgccgaa gagcacgctc atgagttgcg ccgggggaaa
      541 ttccgggtcc ggtcgcaagc gaatgtcgag gtagtggtcc attctagagc cgccggtcac
      601 acgccagaag ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg
      661 acagccccga ggctggagag ggagaagggg acggcggcgc ggc
//
