LOCUS       sequence-161545-         401 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A single vector AAV-Cas9 system containing Cas9 from Staphylococcus 
            aureus (SaCas9) and its sgRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B,
            Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F
  TITLE     In vivo genome editing using Staphylococcus aureus Cas9.
  JOURNAL   Nature. 2015 Apr 1. doi: 10.1038/nature14299.
  PUBMED    25830891
REFERENCE   2  (bases 1 to 401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Apr 1. doi: 10.1038/nature14299."
COMMENT     Sequence Label: 
            pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
FEATURES             Location/Qualifiers
     source          1..401
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     139..158
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        237..312
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
ORIGIN
        1 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag
       61 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
      121 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
      181 ttggctttat atatcttgtg gaaaggacga aacaccggag accacggcag gtctcagttt
      241 tagtactctg gaaacagaat ctactaaaac aaggcaaaat gccgtgttta tctcgtcaac
      301 ttgttggcga gatttttgcg gccgcaggaa cccctagtga tggagttggc cactccctct
      361 ctgcgcgctc gctcgctcac ngaggccggg cgaccaaagg t
//
