LOCUS       sequence-161549-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses murine SRAP domain (250 aa) in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Kweon SM, Zhu B, Chen Y, Aravind L, Xu SY, Feldman DE
  TITLE     Erasure of Tet-Oxidized 5-Methylcytosine by a SRAP Nuclease.
  JOURNAL   Cell Rep. 2017 Oct 10;21(2):482-494. doi: 
            10.1016/j.celrep.2017.09.055.
  PUBMED    29020633
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.09"; journalName: "Cell Rep"; date: 
            "2017-10-10- 10"; volume: "21"; issue: "2"; pages: "482-494"
COMMENT     Sequence Label: pET30a-SRAP
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..32
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             847..864
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 annnanngnc tggtctggtg ccacgcggtt ctggtatgaa accaaaccgc tgctgctaaa
       61 ttcgaacgcc agcacatgga cagcccagat ctgtgcgggc gaacgtcctg tcacttgccc
      121 agagaggttc tcaccagggc ctgcgcctat caggatcggc agggccggcg gcggctcccg
      181 cagtggaggg accccgacaa gtactgcccc tcctacaaca agagcccgca gtccagcagc
      241 cccgtgctgc tctccagact gcactttgag aaggatgcag actcatcaga tcggataatt
      301 attcccatgc gatggggctt agtcccatct tggttcaaag aaagtgatcc ttctaagctg
      361 cagttcaaca ctaccaactg tcgcagtgat accataatgg agaagcagtc attcaaggtt
      421 cctctgggga aaggacggcg gtgtgttgtt ttagcagatg gattctacga gtggcagcgg
      481 tgtcagggaa caaaccagag gcaaccatac ttcatctatt ttcctcaaat caagacagag
      541 aagtcaggtg ggaacgatgc ttcagacagc tctgacaaca aggaaaaggt ctgggacaac
      601 tggaggctgc tgacaatggc agggatcttt gactgctggg aagcgccagg gggagagtgc
      661 ctgtattcct acagcatcat cactgtggat tcctgcagag gtttgagtga catccacagc
      721 aggatgcctg ccatactaga tggagaagaa gcagtctcca aatggctcga ctttggtgag
      781 gtcgccactc aggaagctct gaagctaatc caccccatag acaatatcac cttccatcca
      841 ctcgagcacc accaccacca ccactganat ccggctgcta acaaagcccg aaaggaagct
      901 gagttggctg ctgccaccgc tgancaataa ctagcataac ccttggggcn nctaaacgg
//
