LOCUS       sequence-161581-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Aromatic amino acid inducible expression of alpha pheromone in yeast
            from the mfalpha2 gene .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Williams TC, Nielsen LK, Vickers CE
  TITLE     Engineered quorum sensing using pheromone-mediated cell-to-cell 
            communication in Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2013 Mar 15;2(3):136-49. doi: 10.1021/sb300110b. 
            Epub 2013 Jan 7.
  PUBMED    23656437
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300110b";
            journalName: "ACS Synth Biol"; date: "2013-03-15- 15"; volume: "2"; 
            issue: "3"; pages: "136-49"
COMMENT     Sequence Label: pTCW010
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     56..72
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     56..72
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg gccgctctag
       61 aactagtgga tccagagctc caaccatagt gaacaatccg gtaatgatag ggaaccttgc
      121 ttgcagacag aatcggaaac ggatatagaa acatagccta tttatgaatg aatgtaatat
      181 atggcaaaga gtattatagt atcgcatcat ttgagagaat actttattta aatggcaaaa
      241 aatagttcta tgtgagtgat cgaaaactca actactcaga ttgaaaagaa aataaataaa
      301 actaggtgtt gtatgaacgt gaaagaaatc gagagggttt agaagtagtt tagggtcatt
      361 tttcagtaca ttggttggcc tggcttgagt tgcaaccagt gccaagcatc agcgttggcc
      421 tctctcttgt acattggttg gcctggtctc aaatttaacc agtgccaagc gtcggcaaca
      481 gcctctcttt tcgccaaagt ggtgttttgc tctttttcag ccgcctcagc aatagtggtg
      541 ttgataaaca atagcccact ggcggtagcg ttactgaatg gtaaaaaagc tatgtcatga
      601 tcacctccga aatccaagta tccaataatg gcctcggctg gcacctgagc gatatcttca
      661 tcggaactag cagtgacaga aacggccgct aaaataaaag tgagaaaggt agaaatgaat
      721 ttcatgaatt ctgagtcgat gagagagtgt aattgtggta tgtgtgtatt tttatgttta
      781 cactgggata ttacaggacc aaatcaagct ttttccttta tatatatcaa atatttataa
      841 tgtttcctac cccaatgatg gttcggttat ccgcggcaaa tctcggtaag catcggcaat
      901 gcttccgcga gttaccgctt tttttttttt tattcctttg ttactatcta cggccgtcgg
      961 cggcaatagt atttttgctc acgtgaccga caagggtcca ttttattgcg gtacggctct
     1021 ctcttttttt tttt
//
