LOCUS       sequence-161592-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pheromone mediated destablised-GFP expression in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Williams TC, Nielsen LK, Vickers CE
  TITLE     Engineered quorum sensing using pheromone-mediated cell-to-cell 
            communication in Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2013 Mar 15;2(3):136-49. doi: 10.1021/sb300110b. 
            Epub 2013 Jan 7.
  PUBMED    23656437
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300110b";
            journalName: "ACS Synth Biol"; date: "2013-03-15- 15"; volume: "2"; 
            issue: "3"; pages: "136-49"
COMMENT     Sequence Label: pTCW002
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(823..842)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(824..842)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(852..869)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(852..868)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(861..883)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atcaccatct aattcaacca aaattgggac aacaccagtg aataattctt cacctttaga
       61 catgaattct ttgattttca gaaacttgat ggcttatatc ctgctcttaa aggaaaaaag
      121 ataaaggatg tcgtatccaa atattttaag agcaacacat accttacaaa gccactctta
      181 cattgtcatt taaataaaac caatcccgag aattacgtta tttgtttcaa attacagacg
      241 tttcatgttt gtttcatcat cgcaccaaaa tgagacagcg cgaaaagtga cagtgcaatt
      301 atgccattca atacgtcaaa aagggcatcc tgcagagata caactctgtt ttgaaagggt
      361 ccacatggta aatatggaag caaaagaagg attgaggcat cactgacaac aaaattgtct
      421 tgcagcccaa ttaataaaaa aaaataaagt tatatatata aacaaatacg taaacaaagg
      481 atagaaggcg aactgaatca tcgttcagta gcggctctgg ttgccgttat tgttctgttg
      541 agaaaagttg ccagaaaaat tgaaggattc gtgctgtcca ttggagttct ggtttccgcc
      601 gtattgcggt cttccgtact cattggattg ctgttgacca ttctgttgcg gtctaccata
      661 ctcattagcc tgttgctggc ccccataact cgaatttgaa ttggagttat tgcccaggta
      721 ggaacttgcc atggaagcca aagctgagaa tgaactggag tggccttgtt gctgctgctg
      781 accctgttgt tgatctcgag ggggggcccg gtacccaatt cgccctatag tgagtcgtat
      841 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      901 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
      961 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgg
//
