LOCUS       sequence-161600-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Aromatic amino acid inducible expression of alpha pheromone in yeast
            from the mfalpha1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Williams TC, Nielsen LK, Vickers CE
  TITLE     Engineered quorum sensing using pheromone-mediated cell-to-cell 
            communication in Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2013 Mar 15;2(3):136-49. doi: 10.1021/sb300110b. 
            Epub 2013 Jan 7.
  PUBMED    23656437
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300110b";
            journalName: "ACS Synth Biol"; date: "2013-03-15- 15"; volume: "2"; 
            issue: "3"; pages: "136-49"
COMMENT     Sequence Label: pTCW009
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..67
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     51..67
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(600..611,612..809,810..866))
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYLDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLDKREAEA"
ORIGIN
        1 accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccgc tctagaacta
       61 gtggatccga ttcgattcac attcatcttt ttttagctac taataggata aattatagga
      121 atttataaca aattgaaata tggcaggcag caaaattaaa attgtcttag ttttttagtg
      181 tatagaagtg aatagctata taaagtatgt gtaaagttgg taacggaacg aaaaatagaa
      241 aaggatatta catgggaaaa catgttgttt acggagaaat gaaaagtata ttgtattttg
      301 tacgagctaa aagtacagtg ggaacaaagt cgactttgtt acatctacac tgttgttatc
      361 agtcgggctt agtacattgg ttggccgggt tttaactgca accaatgcca agcttcagcg
      421 tcggcttctc ttttgtacat tggttggcca ggctttagtt gcagccaatg ccaagcttca
      481 gcgtcggctt ctcttttgta cattggttgg ccaggcttta gttgcagcca atgccaagct
      541 tcagcttcgg cttctctctt gtacattggt tggccaggtt ttagttgcaa ccaatgccaa
      601 gcttcagcct ctcttttatc caaagatacc ccttcttctt tagcagcaat gctggcaata
      661 gtagtattta taaacaataa cccgttattt gtgctgttgg aaaatggcaa aacagcaaca
      721 tcgaaatccc cttctaaatc taagtaaccg atgacagctt cagccggaat ttgtgccgtt
      781 tcatcttctg ttgtagtgtt gactggagca gctaatgcgg aggatgctgc gaataaaact
      841 gcagtaaaaa ttgaaggaaa tctcatgaat tctgagtcga tgagagagtg taattgtggt
      901 atgtgtgtat ttttatgttt acactgggat attacaagac caaatcaagc tttttccttt
      961 atatatatca aatatttata atgtttccta ccccaatgat ggttcggtta tccgcggcaa
     1021 atctcggtaa gcatcggcaa tgcttccgcg ag
//
