LOCUS       sequence-161602-        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pheromone mediated alpha pheromone expression in yeast from the 
            mfalpha 1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Williams TC, Nielsen LK, Vickers CE
  TITLE     Engineered quorum sensing using pheromone-mediated cell-to-cell 
            communication in Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2013 Mar 15;2(3):136-49. doi: 10.1021/sb300110b. 
            Epub 2013 Jan 7.
  PUBMED    23656437
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300110b";
            journalName: "ACS Synth Biol"; date: "2013-03-15- 15"; volume: "2"; 
            issue: "3"; pages: "136-49"
COMMENT     Sequence Label: pTCW005
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     57..73
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     57..73
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(606..617,618..815,816..872))
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYLDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLDKREAEA"
ORIGIN
        1 caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta
       61 gaactagtgg atccgattcg attcacattc atcttttttt agctactaat aggataaatt
      121 ataggaattt ataacaaatt gaaatatggc aggcagcaaa attaaaattg tcttagtttt
      181 ttagtgtata gaagtgaata gctatataaa gtatgtgtaa agttggtaac ggaacgaaaa
      241 atagaaaagg atattacatg ggaaaacatg ttgtttacgg agaaatgaaa agtatattgt
      301 attttgtacg agctaaaagt acagtgggaa caaagtcgac tttgttacat ctacactgtt
      361 gttatcagtc gggcttagta cattggttgg ccgggtttta actgcaacca atgccaagct
      421 tcagcgtcgg cttctctttt gtacattggt tggccaggct ttagttgcag ccaatgccaa
      481 gcttcagcgt cggcttctct tttgtacatt ggttggccag gctttagttg cagccaatgc
      541 caagcttcag cttcggcttc tctcttgtac attggttggc caggttttag ttgcaaccaa
      601 tgccaagctt cagcctctct tttatccaaa gatacccctt cttctttagc agcaatgctg
      661 gcaatagtag tatttataaa caataacccg ttatttgtgc tgttggaaaa tggcaaaaca
      721 gcaacatcga aatccccttc taaatctaag taaccgatga cagcttcagc cggaatttgt
      781 gccgtttcat cttctgttgt agtgttgact ggagcagcta atgcggagga tgctgcgaat
      841 aaaactgcag taaaaattga aggaaatctc atgaattctt tgattttcag aaacttgatg
      901 gcttatatcc tgctcttaaa ggaaaaaaga taaaggatgt cgtatccaaa tattttaaga
      961 gcaacacata ccttacaaag ccactcttac attgtcattt aaataaaacc aatcccgaga
     1021 attacgttat ttgtttcaaa ttacagacgt ttcatgtttg ttt
//
