LOCUS       sequence-161605-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pheromone mediated alpha pheromone expression in yeast from the 
            mfalpha 1 gene and FUSJ2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Williams TC, Nielsen LK, Vickers CE
  TITLE     Engineered quorum sensing using pheromone-mediated cell-to-cell 
            communication in Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2013 Mar 15;2(3):136-49. doi: 10.1021/sb300110b. 
            Epub 2013 Jan 7.
  PUBMED    23656437
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300110b";
            journalName: "ACS Synth Biol"; date: "2013-03-15- 15"; volume: "2"; 
            issue: "3"; pages: "136-49"
COMMENT     Sequence Label: pTCW007
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(542..598,599..796,797..808)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYLDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLDKREAEA"
ORIGIN
        1 atagggcgaa ttgggtaccg ggccccccct cgagccctcc ttcaattttt ctggcaactt
       61 ttctcaacag aacaataacg gcaaccagaa ccgctactga acgatgattc agttcgcctt
      121 ctatcctttg ttttcgtatt tgtttatata tataacttta ttttttttta ttaattgggc
      181 tgcaagacaa ttttgttgtc agtgatgcct caatccttct tttgcttcca tatttaccat
      241 gtggaccctt tcaaaacaga gttgtatctc tgcaggatgc cctttttgac gtattgaatg
      301 gcataattgc actgtcactt ttcgagctgt cccattttgg tgcgatgatg gaacaaacat
      361 gaaacgtctg taatttgaaa caaataacgt aattctcggg attggtttta tttaaatgac
      421 aatgtaagag tggctttgta aggtatgtgt tgctcttaaa atatttggat acgacttccg
      481 ttatcttttt tcctttaaga gcaggatata agccaacaag tttctgaaaa tcaaagaatt
      541 catgagattt ccttcaattt ttactgcagt tttattcgca gcatcctccg cattagctgc
      601 tccagtcaac actacaacag aagatgaaac ggcacaaatt ccggctgaag ctgtcatcgg
      661 ttacttagat ttagaagggg atttcgatgt tgctgttttg ccattttcca acagcacaaa
      721 taacgggtta ttgtttataa atactactat tgccagcatt gctgctaaag aagaaggggt
      781 atctttggat aaaagagagg ctgaagcttg gcattggttg caactaaaac ctggccaacc
      841 aatgtacaag agagaagccg aagctgaagc ttggcattgg ctgcaactaa agcctggcca
      901 accaatgtac aaaagagaag ccgacgctga agcttggcat tggctgcaac taaagcctgg
//
