LOCUS       sequence-161606-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pheromone mediated alpha pheromone expression in yeast from the 
            mfalpha 1 gene and FUSJ2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Williams TC, Nielsen LK, Vickers CE
  TITLE     Engineered quorum sensing using pheromone-mediated cell-to-cell 
            communication in Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2013 Mar 15;2(3):136-49. doi: 10.1021/sb300110b. 
            Epub 2013 Jan 7.
  PUBMED    23656437
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300110b";
            journalName: "ACS Synth Biol"; date: "2013-03-15- 15"; volume: "2"; 
            issue: "3"; pages: "136-49"
COMMENT     Sequence Label: pTCW007
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..71
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     55..71
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(604..615,616..813,814..870))
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYLDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLDKREAEA"
ORIGIN
        1 attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgctctaga
       61 actagtggat ccgattcgat tcacattcat ctttttttag ctactaatag gataaattat
      121 aggaatttat aacaaattga aatatggcag gcagcaaaat taaaattgtc ttagtttttt
      181 agtgtataga agtgaatagc tatataaagt atgtgtaaag ttggtaacgg aacgaaaaat
      241 agaaaaggat attacatggg aaaacatgtt gtttacggag aaatgaaaag tatattgtat
      301 tttgtacgag ctaaaagtac agtgggaaca aagtcgactt tgttacatct acactgttgt
      361 tatcagtcgg gcttagtaca ttggttggcc gggttttaac tgcaaccaat gccaagcttc
      421 agcgtcggct tctcttttgt acattggttg gccaggcttt agttgcagcc aatgccaagc
      481 ttcagcgtcg gcttctcttt tgtacattgg ttggccaggc tttagttgca gccaatgcca
      541 agcttcagct tcggcttctc tcttgtacat tggttggcca ggttttagtt gcaaccaatg
      601 ccaagcttca gcctctcttt tatccaaaga taccccttct tctttagcag caatgctggc
      661 aatagtagta tttataaaca ataacccgtt atttgtgctg ttggaaaatg gcaaaacagc
      721 aacatcgaaa tccccttcta aatctaagta accgatgaca gcttcagccg gaatttgtgc
      781 cgtttcatct tctgttgtag tgttgactgg agcagctaat gcggaggatg ctgcgaataa
      841 aactgcagta aaaattgaag gaaatctcat gaattctttg attttcagaa acttgttggc
      901 ttatatcctg ctcttaaagg aaaaaagata acggaagtcg tatccaaata ttttaagagc
      961 aacacatacc ttacaaagcc actcttacat tgtcatttaa ataaaaccaa tcccgagaat
     1021 tacgttattt gtttcaaatt acagacgttt c
//
