LOCUS       sequence-161610-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pheromone mediated alpha pheromone expression in yeast from the 
            mfalpha 2 gene and FUSJ2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Williams TC, Nielsen LK, Vickers CE
  TITLE     Engineered quorum sensing using pheromone-mediated cell-to-cell 
            communication in Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2013 Mar 15;2(3):136-49. doi: 10.1021/sb300110b. 
            Epub 2013 Jan 7.
  PUBMED    23656437
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300110b";
            journalName: "ACS Synth Biol"; date: "2013-03-15- 15"; volume: "2"; 
            issue: "3"; pages: "136-49"
COMMENT     Sequence Label: pTCW008
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..22
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4..22
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     59..75
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     59..75
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cgcaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccgctc
       61 tagaactagt ggatccagag ctccaaccat agtgaacaat ccggtaatga tagggaacct
      121 tgcttgcaga cagaatcgga aacggatata gaaacatagc ctatttatga atgaatgtaa
      181 tatatggcaa agagtattat agtatcgcat catttgagag aatactttat ttaaatggca
      241 aaaaatagtt ctatgtgagt gatcgaaaac tcaactactc agattgaaaa gaaaataaat
      301 aaaactaggt gttgtatgaa cgtgaaagaa atcgagaggg tttagaagta gtttagggtc
      361 atttttcagt acattggttg gcctggcttg agttgcaacc agtgccaagc atcagcgttg
      421 gcctctctct tgtacattgg ttggcctggt ctcaaattta accagtgcca agcgtcggca
      481 acagcctctc ttttcgccaa agtggtgttt tgctcttttt cagccgcctc agcaatagtg
      541 gtgttgataa acaatagccc actggcggta gcgttactga atggtaaaaa agctatgtca
      601 tgatcacctc cgaaatccaa gtatccaata atggcctcgg ctggcacctg agcgatatct
      661 tcatcggaac tagcagtgac agaaacggcc gctaaaataa aagtgagaaa ggtagaaatg
      721 aatttcatga attctttgat tttcagaaac ttgttggctt atatcctgct cttaaaggaa
      781 aaaagataac ggaagtcgta tccaaatatt ttaagagcaa cacatacctt acaaagccac
      841 tcttacattg tcatttaaat aaaaccaatc ccgagaatta cgttatttgt ttcaaattac
      901 agacgtttca tgtttgttcc atcatcgcac caaaatggga cagctcgaaa agtgacagtg
      961 caattatgcc attcaatacg tcaaaaaggg catcctgcag agatacaact ctgttttgaa
     1021 agggtccaca tg
//
