LOCUS       sequence-161611-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning destination vector for phermone mediated expression from the
            FUS1J2 promoter and CYC1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Williams TC, Averesch NJH, Winter G, Plan MR, Vickers CE, Nielsen 
            LK, Kromer JO
  TITLE     Quorum-sensing linked RNA interference for dynamic metabolic pathway
            control in Saccharomyces cerevisiae.
  JOURNAL   Metab Eng. 2015 May;29:124-134. doi: 10.1016/j.ymben.2015.03.008. 
            Epub 2015 Mar 16.
  PUBMED    25792511
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-05"; volume: "29:124-134. doi"; pages: " 
            10.1016/j.ymben.2015.03.008. Epub 2015 Mar 16"
COMMENT     Sequence Label: pTCW012
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(555..571)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(555..571)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      597..844
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(613..631)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(890..910)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(890..908)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(929..945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(929..945)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(942..964)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    953..969
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atagggcgaa ttgggtaccg ggccccccct cgagccctcc ttcaattttt ctggcaactt
       61 ttctcaacag aacaataacg gcaaccagaa ccgctactga acgatgattc agttcgcctt
      121 ctatcctttg ttttcgtatt tgtttatata tataacttta ttttttttta ttaattgggc
      181 tgcaagacaa ttttgttgtc agtgatgcct caatccttct tttgcttcca tatttaccat
      241 gtggaccctt tcaaaacaga gttgtatctc tgcaggatgc cctttttgac gtattgaatg
      301 gcataattgc actgtcactt ttcgagctgt cccattttgg tgcgatgatg gaacaaacat
      361 gaaacgtctg taatttgaaa caaataacgt aattctcggg attggtttta tttaaatgac
      421 aatgtaagag tggctttgta aggtatgtgt tgctcttaaa atatttggat acgacttccg
      481 ttatcttttt tcctttaaga gcaggatata agccaacaag tttctgaaaa tcaaagaatt
      541 cctgcagccc gggggatcca ctagttctag aacaggcccc ttttcctttg tcgatatcat
      601 gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa
      661 ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt
      721 attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacaaac gcgtgtacgc
      781 atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat
      841 ttgcaagctt cgcagtttac actctcatcg tgagctccag cttttgttcc ctttagtgag
      901 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      961 cgctcacaat tccacaca
//
