LOCUS       sequence-161612-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning destination vector for phermone mediated expression from the
            FUS1J2 promoter and CYC1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Williams TC, Averesch NJH, Winter G, Plan MR, Vickers CE, Nielsen 
            LK, Kromer JO
  TITLE     Quorum-sensing linked RNA interference for dynamic metabolic pathway
            control in Saccharomyces cerevisiae.
  JOURNAL   Metab Eng. 2015 May;29:124-134. doi: 10.1016/j.ymben.2015.03.008. 
            Epub 2015 Mar 16.
  PUBMED    25792511
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-05"; volume: "29:124-134. doi"; pages: " 
            10.1016/j.ymben.2015.03.008. Epub 2015 Mar 16"
COMMENT     Sequence Label: pTCW012
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(209..424)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(448..467)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     567..589
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(627..645)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        713..817
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg
       61 cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg
      121 tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag
      181 cacgttcctt atatgtagct ttcgacatga tttatcttcg tttcctgcag gtttttgttc
      241 tgtgcagttg ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta
      301 tatataccaa tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg
      361 aatcaaaaaa atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat
      421 tgaaaaggtg gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
      481 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
      541 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
      601 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
      661 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
      721 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      781 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      841 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      901 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      961 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgt
//
