LOCUS       sequence-161622-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  UBiC and feedback resistant ARO4 expression from pheromone inducible
            FUS1J2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Williams TC, Averesch NJH, Winter G, Plan MR, Vickers CE, Nielsen 
            LK, Kromer JO
  TITLE     Quorum-sensing linked RNA interference for dynamic metabolic pathway
            control in Saccharomyces cerevisiae.
  JOURNAL   Metab Eng. 2015 May;29:124-134. doi: 10.1016/j.ymben.2015.03.008. 
            Epub 2015 Mar 16.
  PUBMED    25792511
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-05"; volume: "29:124-134. doi"; pages: " 
            10.1016/j.ymben.2015.03.008. Epub 2015 Mar 16"
COMMENT     Sequence Label: pTCW015
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(53..300)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     266..284
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     326..342
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     326..342
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tcactaaagg gaacaaaagc tggagacgat gagagtgtaa actgcgaagc ttgcaaatta
       61 aagccttcga gcgtcccaaa accttctcaa gcaaggtttt cagtataatg ttacatgcgt
      121 acacgcgttt gtacagaaaa aaaagaaaaa tttgaaatat aaataacgtt cttaatacta
      181 acataactat aaaaaaataa atagggacct agacttcagg ttgtctaact ccttcctttt
      241 cggttagagc ggatgtgggg ggagggcgtg aatgtaagcg tgacataact aattacatga
      301 tatcgacaaa ggaaaagggg cctgttctag aactagtgga tcccccgggc tatttcttgt
      361 taacttctct tctttgtctg acagcagcag ccaatttcct caagacgtct tcagtagttt
      421 cccaacctat acaagcatca gtgatggaaa caccatattt caagccggct ttaccttcgg
      481 ctgggatgcc ttggttacct tcgttgatgt ttgattcaat catgacaccg gtaatggcgt
      541 tttcaccgtt agcgatttgc tcacaaacaa cgtcattgac ctttggttgg tttctgaaat
      601 ccttattgga gttaccgtga gagtagtcaa tcattagacc gttggaaccg gcaggcaatt
      661 gagccttagc ttctgcaacg gacttagcgt cgtagttggt acccttttta ccacctctta
      721 gaataacgaa gcagtgttcg ttacccttag tagtggtgat agcagcaaca ccatgcttag
      781 taacacccat gaaatggtga gaatgagcag cggcttgaca agcatccaca gcaacattta
      841 aggtaccatc ggtaccgttc ttgaaaccaa ctgggaaaga caaaccggag gccaattctc
      901 tgtgcagttg agattcggtg gttctggcac caatggcacc gaaggagacc aaatcagcca
      961 agtat
//
