LOCUS       sequence-161639-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 
            in the array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Vad-Nielsen J, Lin L, Bolund L, Nielsen AL, Luo Y
  TITLE     Golden Gate Assembly of CRISPR gRNA expression array for 
            simultaneously targeting multiple genes.
  JOURNAL   Cell Mol Life Sci. 2016 May 13.
  PUBMED    27178736
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2016 May 13."
COMMENT     Sequence Label: pMA-SpCas9-g7
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(192..296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     597..619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     611..628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     612..628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     639..658
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        639..657
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     722..742
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
       61 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      301 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
      361 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
      421 aatcaaaaga atagaccgag atagggttga gtggccgcta cagggcgctc ccattcgcca
      481 ttcaggctgc gcaactgttg ggaagggcgt ttcggtgcgg gcctcttcgc tattacgcca
      541 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
      601 gtcacgacgt tgtaaaacga cggccagtga gcgcgacgta atacgactca ctatagggcg
      661 aattggcgga aggccgtcaa ggccacgtgt cttgtccaga gctcggatcc ggtctcacgg
      721 tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
      781 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
      841 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
      901 tatgcttac
//
