LOCUS       sequence-161653-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive TEF1 promoter mediated expression of the UBiC gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Williams TC, Peng B, Vickers CE, Nielsen LK
  TITLE     The Saccharomyces cerevisiae pheromone-response is a metabolically 
            active stationary phase for bio-production.
  JOURNAL   Metab Eng Commun. 2016 May 11;3:142-152. doi: 
            10.1016/j.meteno.2016.05.001. eCollection 2016 Dec.
  PUBMED    29468120
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2016-05-11"; volume: "3:142-152. doi"; pages: " 
            10.1016/j.meteno.2016.05.001. eCollection 2016 Dec"
COMMENT     Sequence Label: PHBA01
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        48..454
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(972..988)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(972..988)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1029..1047)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ctcgaggcac acaccatagc
       61 ttcaaaatgt ttctactcct tttttactct tccagatttt ctcggactcc gcgcatcgcc
      121 gtaccacttc aaaacaccca agcacagcat actaaatttc ccctctttct tcctctaggg
      181 tgtcgttaat tacccgtact aaaggtttgg aaaagaaaaa agagaccgcc tcgtttcttt
      241 ttcttcgtcg aaaaaggcaa taaaaatttt tatcacgttt ctttttcttg aaaatttttt
      301 tttttgattt ttttctcttt cgatgacctc ccattgatat ttaagttaat aaacggtctt
      361 caatttctca agtttcagtt tcatttttct tgttctatta caactttttt tacttcttgc
      421 tcattagaaa gaaagcatag caatctaatc taagttttaa ttacaaatgt cccatccagc
      481 tttgacacaa ttgagagctt tgagatactg caaagaaatt ccagcattgg atccacaatt
      541 attggactgg ttgttgttgg aagattccat gactaagaga ttcgaacaac aaggtaagac
      601 cgtttccgtt accatgatta gagaaggttt cgtcgaacaa aacgaaatcc cagaagaatt
      661 gcctttgttg ccaaaagaat ctagatactg gttgagagaa attttgttgt gcgctgatgg
      721 tgaaccttgg ttggctggta gaactgttgt tccagtttct actttgtctg gtccagaatt
      781 ggcattgcaa aaattgggta aaactccatt gggtagatac ttgttcactt cttctacctt
      841 gaccagagac ttcattgaaa ttggtagaga tgctggtttg tggggtagaa gatcaagatt
      901 gagattgtct ggtaaacctt tgttgttgac cgaattattc ttgccagctt ccccattata
      961 ctgacccggg ggatccacta gttctagaac agcccctttt cctttgtcga tatcatgtaa
     1021 ttagttatgt cacgcttaca ttcacgccc
//
