LOCUS       sequence-161654-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive TEF1 promoter mediated expression of the UBiC gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Williams TC, Peng B, Vickers CE, Nielsen LK
  TITLE     The Saccharomyces cerevisiae pheromone-response is a metabolically 
            active stationary phase for bio-production.
  JOURNAL   Metab Eng Commun. 2016 May 11;3:142-152. doi: 
            10.1016/j.meteno.2016.05.001. eCollection 2016 Dec.
  PUBMED    29468120
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2016-05-11"; volume: "3:142-152. doi"; pages: " 
            10.1016/j.meteno.2016.05.001. eCollection 2016 Dec"
COMMENT     Sequence Label: PHBA01
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..31
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15..31
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(549..955)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 gaaaaggggc ctgttctaga actagtggat cccccgggtc agtataatgg ggaagctggc
       61 aagaataatt cggtcaacaa caaaggttta ccagacaatc tcaatcttga tcttctaccc
      121 cacaaaccag catctctacc aatttcaatg aagtctctgg tcaaggtaga agaagtgaac
      181 aagtatctac ccaatggagt tttacccaat ttttgcaatg ccaattctgg accagacaaa
      241 gtagaaactg gaacaacagt tctaccagcc aaccaaggtt caccatcagc gcacaacaaa
      301 atttctctca accagtatct agattctttt ggcaacaaag gcaattcttc tgggatttcg
      361 ttttgttcga cgaaaccttc tctaatcatg gtaacggaaa cggtcttacc ttgttgttcg
      421 aatctcttag tcatggaatc ttccaacaac aaccagtcca ataattgtgg atccaatgct
      481 ggaatttctt tgcagtatct caaagctctc aattgtgtca aagctggatg ggacatttgt
      541 aattaaaact tagattagat tgctatgctt tctttctaat gagcaagaag taaaaaaagt
      601 tgtaatagaa caagaaaaat gaaactgaaa cttgagaaat tgaagaccgt ttattaactt
      661 aaatatcaat gggaggtcat cgaaagagaa aaaaatcaaa aaaaaaaatt ttcaagaaaa
      721 agaaacgtga taaaaatttt tattgccttt ttcgacgaag aaaaagaaac gaggcggtct
      781 cttttttctt ttccaaacct ttagtacggg taattaacga caccctagag gaagaaagag
      841 gggaaattta gtatgctgtg cttgggtgtt ttgaagtggt acggcgatgc gcggagtccg
      901 agaaaatctg gaagagtaaa aaaggagtag aaacattttg aagctatggt gtgtgcctcg
      961 aggtcgacct cgaggggggg cccggtaccc aattcgccct a
//
