LOCUS       sequence-161660-        5094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing BRAF, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_BRAF_p.P367R
FEATURES             Location/Qualifiers
     source          1..5094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2199..2298)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2315..2334)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2316..2334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2339..2355)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2339..2355)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2786..3577
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3670..4258
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4159..4178
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4412..4429
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4588..4615)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4707..4793)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4842..4864
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4856..4873
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4857..4873
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4889..4988
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      4989..4998
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcttcggct gcggaccctg ccattccgga ggaggtgtgg aatatcaaac aaatgattaa
       61 gttgacacag gaacatatag aggccctatt ggacaaattt ggtggggagc ataatccacc
      121 atcaatatat ctggaggcct atgaagaata caccagcaag ctagatgcac tccaacaaag
      181 agaacaacag ttattggaat ctctggggaa cggaactgat ttttctgttt ctagctctgc
      241 atcaatggat accgttacat cttcttcctc ttctagcctt tcagtgctac cttcatctct
      301 ttcagttttt caaaatccca cagatgtggc acggagcaac cccaagtcac cacaaaaacc
      361 tatcgttaga gtcttcctgc ccaacaaaca gaggacagtg gtacctgcaa ggtgtggagt
      421 tacagtccga gacagtctaa agaaagcact gatgatgaga ggtctaatcc cagagtgctg
      481 tgctgtttac agaattcagg atggagagaa gaaaccaatt ggttgggaca ctgatatttc
      541 ctggcttact ggagaagaat tgcatgtgga agtgttggag aatgttccac ttacaacaca
      601 caactttgta cgaaaaacgt ttttcacctt agcattttgt gacttttgtc gaaagctgct
      661 tttccagggt ttccgctgtc aaacatgtgg ttataaattt caccagcgtt gtagtacaga
      721 agttccactg atgtgtgtta attatgacca acttgatttg ctgtttgtct ccaagttctt
      781 tgaacaccac ccaataccac aggaagaggc gtccttagca gagactgccc taacatctgg
      841 atcatcccct tccgcacccg cctcggactc tattgggccc caaattctca ccagtccgtc
      901 tccttcaaaa tccattccaa ttccacagcc cttccgacca gcagatgaag atcatcgaaa
      961 tcaatttggg caacgagacc gatcctcatc agctcgcaat gtgcatataa acacaataga
     1021 acctgtcaat attgatgact tgattagaga ccaaggattt cgtggtgatg gaggatcaac
     1081 cacaggtttg tctgctaccc cccctgcctc attacctggc tcactaacta acgtgaaagc
     1141 cttacagaaa tctccaggac ctcagcgaga aaggaagtca tcttcatcct cagaagacag
     1201 gaatcgaatg aaaacacttg gtagacggga ctcgagtgat gattgggaga ttcctgatgg
     1261 gcagattaca gtgggacaaa gaattggatc tggatcattt ggaacagtct acaagggaaa
     1321 gtggcatggt gatgtggcag tgaaaatgtt gaatgtgaca gcacctacac ctcagcagtt
     1381 acaagccttc aaaaatgaag taggagtact caggaaaaca cgacatgtga atatcctact
     1441 cttcatgggc tattccacaa agccacaact ggctattgtt acccagtggt gtgagggctc
     1501 cagcttgtat caccatctcc atatcattga gaccaaattt gagatgatca aacttataga
     1561 tattgcacga cagactgcac agggcatgga ttacttacac gccaagtcaa tcatccacag
     1621 agacctcaag agtaataata tatttcttca tgaagacctc acagtaaaaa taggtgattt
     1681 tggtctagct acagtgaaat ctcgatggag tgggtcccat cagtttgaac agttgtctgg
     1741 atccattttg tggatggcac cagaagtcat cagaatgcaa gataaaaatc catacagctt
     1801 tcagtcagat gtatatgcat ttggaattgt tctgtatgaa ttgatgactg gacagttacc
     1861 ttattcaaac atcaacaaca gggaccagat aatttttatg gtgggacgag gatacctgtc
     1921 tccagatctc agtaaggtac ggagtaactg tccaaaagcc atgaagagat taatggcaga
     1981 gtgcctcaaa aagaaaagag atgagagacc actctttccc caaattctcg cctctattga
     2041 gctgctggcc cgctcattgc caaaaattca ccgcagtgca tcagaaccct ccttgaatcg
     2101 ggctggtttc caaacagagg attttagtct atatgcttgt gcttctccaa aaacacccat
     2161 ccaggcaggg ggatatggtg cgtttcctgt ccactgattg ccaactttct tgtacaaagt
     2221 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2281 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     2341 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     2401 agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc
     2461 gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     2521 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     2581 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     2641 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     2701 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     2761 tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca
     2821 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     2881 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     2941 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     3001 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     3061 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     3121 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     3181 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     3241 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     3301 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     3361 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     3421 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     3481 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     3541 cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa
     3601 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3661 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3721 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3781 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3841 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3901 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3961 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4021 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4081 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4141 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4201 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4261 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4321 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4381 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     4441 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     4501 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
     4561 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
     4621 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
     4681 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
     4741 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
     4801 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
     4861 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
     4921 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
     4981 agttggcacc atggcggcgc tgagcggtgg cggtggtggc ggcgcggagc cgggccaggc
     5041 tctgttcaac ggggacatgg agcccgaggc cggcgccggc gccggcgccg cggc
//
