LOCUS       sequence-161662-        5139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CTNNB1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_CTNNB1_p.G367V
FEATURES             Location/Qualifiers
     source          1..5139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     191..210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     444..461
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(620..647)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(739..825)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     874..896
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     888..905
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     889..905
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    921..1020
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1023..3365
                     /codon_start=1
                     /gene="Homo sapiens CTNNB1"
                     /product="human cadherin-associated protein that also plays
                     a role in the canonical Wnt signaling pathway"
                     /label=beta-catenin
                     /note="also known as catenin beta-1"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALVLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     protein_bind    complement(3370..3469)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3486..3505)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3487..3505)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3510..3526)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3510..3526)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3957..4748
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4841..5139,1..290)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
       61 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      121 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      181 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      241 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      301 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      361 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      421 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      481 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      541 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
      601 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
      661 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
      721 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
      781 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
      841 ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt aaaacgacgg
      901 ccagtcttaa gctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt
      961 tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagttggca
     1021 ccatggctac tcaagctgat ttgatggagt tggacatggc catggaacca gacagaaaag
     1081 cggctgttag tcactggcag caacagtctt acctggactc tggaatccat tctggtgcca
     1141 ctaccacagc tccttctctg agtggtaaag gcaatcctga ggaagaggat gtggatacct
     1201 cccaagtcct gtatgagtgg gaacagggat tttctcagtc cttcactcaa gaacaagtag
     1261 ctgatattga tggacagtat gcaatgactc gagctcagag ggtacgagct gctatgttcc
     1321 ctgagacatt agatgagggc atgcagatcc catctacaca gtttgatgct gctcatccca
     1381 ctaatgtcca gcgtttggct gaaccatcac agatgctgaa acatgcagtt gtaaacttga
     1441 ttaactatca agatgatgca gaacttgcca cacgtgcaat ccctgaactg acaaaactgc
     1501 taaatgacga ggaccaggtg gtggttaata aggctgcagt tatggtccat cagctttcta
     1561 aaaaggaagc ttccagacac gctatcatgc gttctcctca gatggtgtct gctattgtac
     1621 gtaccatgca gaatacaaat gatgtagaaa cagctcgttg taccgctggg accttgcata
     1681 acctttccca tcatcgtgag ggcttactgg ccatctttaa gtctggaggc attcctgccc
     1741 tggtgaaaat gcttggttca ccagtggatt ctgtgttgtt ttatgccatt acaactctcc
     1801 acaacctttt attacatcaa gaaggagcta aaatggcagt gcgtttagct ggtgggctgc
     1861 agaaaatggt tgccttgctc aacaaaacaa atgttaaatt cttggctatt acgacagact
     1921 gccttcaaat tttagcttat ggcaaccaag aaagcaagct catcatactg gctagtggtg
     1981 gaccccaagc tttagtaaat ataatgagga cctatactta cgaaaaacta ctgtggacca
     2041 caagcagagt gctgaaggtg ctatctgtct gctctagtaa taagccggct attgtagaag
     2101 ctggtggaat gcaagcttta gtacttcacc tgacagatcc aagtcaacgt cttgttcaga
     2161 actgtctttg gactctcagg aatctttcag atgctgcaac taaacaggaa gggatggaag
     2221 gtctccttgg gactcttgtt cagcttctgg gttcagatga tataaatgtg gtcacctgtg
     2281 cagctggaat tctttctaac ctcacttgca ataattataa gaacaagatg atggtctgcc
     2341 aagtgggtgg tatagaggct cttgtgcgta ctgtccttcg ggctggtgac agggaagaca
     2401 tcactgagcc tgccatctgt gctcttcgtc atctgaccag ccgacaccaa gaagcagaga
     2461 tggcccagaa tgcagttcgc cttcactatg gactaccagt tgtggttaag ctcttacacc
     2521 caccatccca ctggcctctg ataaaggcta ctgttggatt gattcgaaat cttgcccttt
     2581 gtcccgcaaa tcatgcacct ttgcgtgagc agggtgccat tccacgacta gttcagttgc
     2641 ttgttcgtgc acatcaggat acccagcgcc gtacgtccat gggtgggaca cagcagcaat
     2701 ttgtggaggg ggtccgcatg gaagaaatag ttgaaggttg taccggagcc cttcacatcc
     2761 tagctcggga tgttcacaac cgaattgtta tcagaggact aaataccatt ccattgtttg
     2821 tgcagctgct ttattctccc attgaaaaca tccaaagagt agctgcaggg gtcctctgtg
     2881 aacttgctca ggacaaggaa gctgcagaag ctattgaagc tgagggagcc acagctcctc
     2941 tgacagagtt acttcactct aggaatgaag gtgtggcgac atatgcagct gctgttttgt
     3001 tccgaatgtc tgaggacaag ccacaagatt acaagaaacg gctttcagtt gagctgacca
     3061 gctctctctt cagaacagag ccaatggctt ggaatgagac tgctgatctt ggacttgata
     3121 ttggtgccca gggagaaccc cttggatatc gccaggatga tcctagctat cgttcttttc
     3181 actctggtgg atatggccag gatgccttgg gtatggaccc catgatggaa catgagatgg
     3241 gtggccacca ccctggtgct gactatccag ttgatgggct gccagatctg gggcatgccc
     3301 aggacctcat ggatgggctg cctccaggtg acagcaatca gctggcctgg tttgatactg
     3361 acctgtgatt gccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
     3421 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
     3481 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     3541 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct
     3601 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
     3661 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     3721 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     3781 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     3841 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     3901 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga
     3961 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     4021 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     4081 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     4141 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     4201 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     4261 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     4321 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     4381 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     4441 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     4501 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     4561 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     4621 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     4681 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     4741 gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     4801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4981 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5101 gtcttaccgg gttggactca agacgatagt taccggata
//
