LOCUS       sequence-161667-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G-protein independent version of optoB2 with point mutations at 
            L72F,Y136G,Y224A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Siuda ER, McCall JG, Al-Hasani R, Shin G, Il Park S, Schmidt MJ, 
            Anderson SL, Planer WJ, Rogers JA, Bruchas MR
  TITLE     Optodynamic simulation of beta-adrenergic receptor signalling.
  JOURNAL   Nat Commun. 2015 Sep 28;6:8480. doi: 10.1038/ncomms9480.
  PUBMED    26412387
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-28"; volume: "6:8480. doi"; pages: " 
            10.1038/ncomms9480"
COMMENT     Sequence Label: pcDNA3-optoB2-LYY-YFP
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             114..152
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttgccaccat gaacggaaca gagggcccaa
      121 acttttacgt tcccttctcc aataagactg gggtcgtgag aagcccattt gaggcgcctc
      181 aatactacct tgctgagccg tggcagtttt ctatgctcgc tgcttacatg ttcttgctga
      241 tcatgctggg gttccctatc aatttcctga cgctgtacgt tatagcaaag ttcgaacgcc
      301 tccaaaccgt gtttaactac atactcctta acctcgcggt tgccgacctc ttcatggttt
      361 tcgggggttt caccaccacc ctctacacct cccttcacgg ctacttcgtg ttcggcccta
      421 ccggatgcaa tctggaaggc tttttcgcaa cgctgggggg ggagattgcc ctttggagcc
      481 tggtggtctt ggccatagag aggggcgtgg tggtcacatc cccattcaag taccagagtt
      541 tgcttacaaa gaacaaggct atcatggggg tcgccttcac atgggtgatg gcgctggctt
      601 gcgctgcccc accgctggta ggctggtccc ggtatattcc ggagggaatg cagtgcagtt
      661 gtgggatcga ctactacacc ccacacgaag agactaacaa cgagtctttt gtgatttata
      721 tgttcgtggt ccacttcatc atccccctga tagtgatctt tttctgtgcc ggcagggtgt
      781 tccaggtcgc caaaaggcag ctccagaaga tcgacaaaag cgaaggccgc tttcacagcc
      841 ccaatcttgg acaggttgaa caggacggca ggtcagggca cgggctgcga cgcagttcta
      901 agttctgcct gaaggaacat aaggccttga gaatggtgat catcatggta atcgccttcc
      961 tgatatgctg gcttccatac gctggcgtgg ctttttatat attcacgcac caggggtcag
     1021 attttgggcc tatctttatg accatacctg ctttcttcgc taagac
//
