LOCUS       sequence-161668-        5139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CTNNB1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_CTNNB1_p.V600G
FEATURES             Location/Qualifiers
     source          1..5139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             124..915
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1008..1596
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1497..1516
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1750..1767
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1926..1953)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2045..2131)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2180..2202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2194..2211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2195..2211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2227..2326
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2329..4671
                     /codon_start=1
                     /gene="Homo sapiens CTNNB1"
                     /product="human cadherin-associated protein that also plays
                     a role in the canonical Wnt signaling pathway"
                     /label=beta-catenin
                     /note="also known as catenin beta-1"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFGQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     protein_bind    complement(4676..4775)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4792..4811)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4793..4811)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4816..4832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4816..4832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
       61 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
      121 attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
      181 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
      241 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
      301 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
      361 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
      421 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
      481 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
      541 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
      601 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
      661 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
      721 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
      781 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
      841 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
      901 gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg
      961 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1021 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1081 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1141 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1201 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1261 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1321 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1381 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1441 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1501 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1561 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     1621 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     1681 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     1741 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     1801 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     1861 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     1921 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     1981 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     2041 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     2101 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     2161 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     2221 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
     2281 gagcaatgct tttttataat gccaactttg tacaaaaaag ttggcaccat ggctactcaa
     2341 gctgatttga tggagttgga catggccatg gaaccagaca gaaaagcggc tgttagtcac
     2401 tggcagcaac agtcttacct ggactctgga atccattctg gtgccactac cacagctcct
     2461 tctctgagtg gtaaaggcaa tcctgaggaa gaggatgtgg atacctccca agtcctgtat
     2521 gagtgggaac agggattttc tcagtccttc actcaagaac aagtagctga tattgatgga
     2581 cagtatgcaa tgactcgagc tcagagggta cgagctgcta tgttccctga gacattagat
     2641 gagggcatgc agatcccatc tacacagttt gatgctgctc atcccactaa tgtccagcgt
     2701 ttggctgaac catcacagat gctgaaacat gcagttgtaa acttgattaa ctatcaagat
     2761 gatgcagaac ttgccacacg tgcaatccct gaactgacaa aactgctaaa tgacgaggac
     2821 caggtggtgg ttaataaggc tgcagttatg gtccatcagc tttctaaaaa ggaagcttcc
     2881 agacacgcta tcatgcgttc tcctcagatg gtgtctgcta ttgtacgtac catgcagaat
     2941 acaaatgatg tagaaacagc tcgttgtacc gctgggacct tgcataacct ttcccatcat
     3001 cgtgagggct tactggccat ctttaagtct ggaggcattc ctgccctggt gaaaatgctt
     3061 ggttcaccag tggattctgt gttgttttat gccattacaa ctctccacaa ccttttatta
     3121 catcaagaag gagctaaaat ggcagtgcgt ttagctggtg ggctgcagaa aatggttgcc
     3181 ttgctcaaca aaacaaatgt taaattcttg gctattacga cagactgcct tcaaatttta
     3241 gcttatggca accaagaaag caagctcatc atactggcta gtggtggacc ccaagcttta
     3301 gtaaatataa tgaggaccta tacttacgaa aaactactgt ggaccacaag cagagtgctg
     3361 aaggtgctat ctgtctgctc tagtaataag ccggctattg tagaagctgg tggaatgcaa
     3421 gctttaggac ttcacctgac agatccaagt caacgtcttg ttcagaactg tctttggact
     3481 ctcaggaatc tttcagatgc tgcaactaaa caggaaggga tggaaggtct ccttgggact
     3541 cttgttcagc ttctgggttc agatgatata aatgtggtca cctgtgcagc tggaattctt
     3601 tctaacctca cttgcaataa ttataagaac aagatgatgg tctgccaagt gggtggtata
     3661 gaggctcttg tgcgtactgt ccttcgggct ggtgacaggg aagacatcac tgagcctgcc
     3721 atctgtgctc ttcgtcatct gaccagccga caccaagaag cagagatggc ccagaatgca
     3781 gttcgccttc actatggact accagttgtg gttaagctct tacacccacc atcccactgg
     3841 cctctgataa aggctactgt tggattgatt cgaaatcttg ccctttgtcc cgcaaatcat
     3901 gcacctttgc gtgagcaggg tgccattcca cgactagttc agttgcttgt tcgtgcacat
     3961 caggataccc agcgccgtac gtccatgggt gggacacagc agcaatttgt ggagggggtc
     4021 cgcatggaag aaatagttga aggttgtacc ggagcccttc acatcctagc tcgggatgtt
     4081 cacaaccgaa ttgttatcag aggactaaat accattccat tgtttgggca gctgctttat
     4141 tctcccattg aaaacatcca aagagtagct gcaggggtcc tctgtgaact tgctcaggac
     4201 aaggaagctg cagaagctat tgaagctgag ggagccacag ctcctctgac agagttactt
     4261 cactctagga atgaaggtgt ggcgacatat gcagctgctg ttttgttccg aatgtctgag
     4321 gacaagccac aagattacaa gaaacggctt tcagttgagc tgaccagctc tctcttcaga
     4381 acagagccaa tggcttggaa tgagactgct gatcttggac ttgatattgg tgcccaggga
     4441 gaaccccttg gatatcgcca ggatgatcct agctatcgtt cttttcactc tggtggatat
     4501 ggccaggatg ccttgggtat ggaccccatg atggaacatg agatgggtgg ccaccaccct
     4561 ggtgctgact atccagttga tgggctgcca gatctggggc atgcccagga cctcatggat
     4621 gggctgcctc caggtgacag caatcagctg gcctggtttg atactgacct gtgattgcca
     4681 actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     4741 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     4801 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     4861 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac
     4921 agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg
     4981 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     5041 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     5101 gtggcggttt tcatggcttg ttatgactgt ttttttggg
//
