LOCUS       sequence-161669-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G-protein independent version of optoB2 with point mutations at 
            L72F,Y136G,Y224A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Siuda ER, McCall JG, Al-Hasani R, Shin G, Il Park S, Schmidt MJ, 
            Anderson SL, Planer WJ, Rogers JA, Bruchas MR
  TITLE     Optodynamic simulation of beta-adrenergic receptor signalling.
  JOURNAL   Nat Commun. 2015 Sep 28;6:8480. doi: 10.1038/ncomms9480.
  PUBMED    26412387
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-28"; volume: "6:8480. doi"; pages: " 
            10.1038/ncomms9480"
COMMENT     Sequence Label: pcDNA3-optoB2-LYY-YFP
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     128..147
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     387..408
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
       61 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
      121 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
      181 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa
      241 gcacttcagg ccgtagccga aggtggtcac gagggtgggc cagggcacgg gcagcttgcc
      301 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
      361 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
      421 cccggtgaac agctcctcgc ccttgctcac catctcgagt gcggccgcgg ccggagcgac
      481 ctgggaagtc tccagggggc tgtcgttggt agagcaattg cgtccctgtg aatcaagaga
      541 gagactcgga actgttccct gacaattcac gaagctctca gtgcctgggg gatcttcaca
      601 aagccgctcg ctctcttttt cctggcccag ctggcagcca ctggcttcgc ccatataatc
      661 agtctttccg ttgctgttag atgaatagcc atttccgtac gctttggaag agctgcgtct
      721 gagacagagc aattcctgga aggcaattct gaattgtttg ttcatcatga tgtatatcac
      781 tgggttatac accgcactcg tcttagcgaa gaaagcaggt atggtcataa agataggccc
      841 aaaatctgac ccctggtgcg tgaatatata aaaagccacg ccagcgtatg gaagccagca
      901 tatcaggaag gcgattacca tgatgatcac cattctcaag gccttatgtt ccttcaggca
      961 gaacttagaa ctgcgtcgca gcccgtgccc tgacctgccg tcctgttcaa cctgtccaag
     1021 attggggctg tgaaagcggc cttcgctttt gtcgatcttc tggagctgcc ttttggcgac
     1081 ctggaa
//
