LOCUS       sequence-161673-        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert
            into the genome using the Piggibac system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Cho WK, Jayanth N, English BP, Inoue T, Andrews JO, Conway W, Grimm 
            JB, Spille JH, Lavis LD, Lionnet T, Cisse II
  TITLE     RNA Polymerase II cluster dynamics predict mRNA output in living 
            cells.
  JOURNAL   Elife. 2016 May 3;5. pii: e13617. doi: 10.7554/eLife.13617.
  PUBMED    27138339
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-05-3"; volume: "5. pii: e13617. doi"; pages: " 
            10.7554/eLife.13617"
COMMENT     Sequence Label: PB53x EF1-Dendra2-RPB1Amr
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    277..329
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      881..890
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcaccgagaa gctgcacgtg cgcgacggcc tgctggtggg caacatcaac atggccctgc
       61 tgctggaggg cggcggccac tacctgtgcg acttcaagac cacctacaag gccaagaagg
      121 tggtgcagct gcccgacgcc cacttcgtgg accaccgcat cgagatcctg ggcaacgaca
      181 gcgactacaa caaggtgaag ctgtacgagc acgccgtggc ccgctacagc cccctgccca
      241 gccaggtgtg gtccggcgac agcggcgtgt acaagactcg agctcaagct tcgaattctg
      301 cagtcgacgg taccgcgggc ccgggatcca ccggatctag catgcacggg ggtggccccc
      361 cctcggggga cagcgcatgc ccgctgcgca ccatcaagag agtccagttc ggagtcctga
      421 gtccggatga actgaagcga atgtctgtga cggagggtgg catcaaatac ccagagacga
      481 ctgagggagg ccgccccaag cttggggggc tgatggaccc gaggcagggg gtgattgagc
      541 ggactggccg ctgccaaaca tgtgcaggaa acatgacaga gtgtcctggc cactttggcc
      601 acattgaact ggccaagcct gtgtttcacg tgggcttcct ggtgaagaca atgaaagttt
      661 tgcgctgtgt ctgcttcttc tgctccaaac tgcttgtgga ctctaacaac ccaaagatca
      721 aggatatcct ggctaagtcc aagggacagc ccaagaagcg gctcacacat gtctacgacc
      781 tttgcaaggg caaaaacata tgcgagggtg gggaggagat ggacaacaag ttcggtgtgg
      841 aacaacctga gggtgacgag gatctgacca aagaaaaggg ccatggtggc tgtgggcggt
      901 accagcccag gatccggcgt tctggcctag agctgtatgc ggaatggaag cacgttaatg
      961 aggactctca ggagaagaag atcctgctga gtccagagcg agtgcatga
//
