LOCUS       sequence-161674-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nadV from Shewanella oneidensis in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Marinescu GC, Popescu RG, Stoian G, Dinischiotu A
  TITLE     beta-nicotinamide mononucleotide (NMN) production in Escherichia 
            coli.
  JOURNAL   Sci Rep. 2018 Aug 16;8(1):12278. doi: 10.1038/s41598-018-30792-0.
  PUBMED    30115969
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-08-16"; volume: "8(1):12278. doi"; pages: " 
            10.1038/s41598-018-30792-0"
COMMENT     Sequence Label: pET28a-soNadV
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     67..86
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        67..85
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    86..110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             125..147
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg
       61 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
      121 taattttgtt taactttaag aaggagatat accatggctt atctgaaccc ggtgacggca
      181 atcgacggct acaaagttga ccatcgccgc caatacccgg acaataccca agttatcttc
      241 tcaaacctga cggcgcgtaa aagccgtcgc ggctataccg atcagatggt gtttttcggt
      301 ctgcaatact tcatcaaaca ttacctgatc gatagttgga atcgcgactt tttccagcaa
      361 ccgaaagaac aagtgatttg ccaattctcc cgtcgcatca acaattatct gggcccgaac
      421 aatgttggta cgcagcatat tgaagaactg cacgatctgg gctacctgcc gattaaaatc
      481 atggccctgc cggaaggtag cgtttatccg ctgaaagtcc cgtgcctgat tctgtacaac
      541 accgatgaac gtttcttttg gctgacgaac tatctggaaa ccatcctgag cgccaatgtg
      601 tggggcatgt gtacgtctgc gaccacggcc ctgcagtatc gtaaaatttt tgaagcgtac
      661 gccctggaaa ccgatggcga catcgcattc gttgattggc aaggtcatga ctttagcttc
      721 cgcggcatgt acggtgtcga agcggccatt atgagtggcg cagctcacct gctgtccttt
      781 accggtacgg ataccattcc ggcaatcgac ttcctggaac agtattacct ggctgattct
      841 gacaaagaac tggtcggcgg ttcagtgccg gcgaccgaac actcggttat gtgtgccggc
      901 ggtatggaaa atgaactgga aacgtttcgt cgcctgattg aagatatcta tccgaccggc
      961 attgt
//
