LOCUS       sequence-161676-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nadV from Haemophilus ducreyi in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Marinescu GC, Popescu RG, Dinischiotu A
  TITLE     Size Exclusion Chromatography Method for Purification of 
            Nicotinamide Mononucleotide (NMN) from Bacterial Cells.
  JOURNAL   Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.1038/s41598-018-22806-8.
  PUBMED    29535407
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-03-13"; volume: "8(1):4433. doi"; pages: " 
            10.1038/s41598-018-22806-8"
COMMENT     Sequence Label: pET28a-hdNadV
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(104..151)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     147..165
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(218..235)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
       61 ggcgcgtccc attcgccaat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
      121 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
      181 cagcttcctt tcgggctttg ttagcagccg gatctcagtg gtggtggtgg tggtgctcga
      241 gtcacagggt cgtgcggcta accagcaggt tctgacggat ttcatcaaag tcggtctgac
      301 gcagcagttt accatcttcg aacagcagtt ccagcaggtc atctgaaaag tcatcggcgg
      361 acgtcaggcc gtccacatag gtatcgcggc tcagaacttt cacacgacct ttctgcgatt
      421 ttttgaagcc gtcatcggtt ttcgggtttt tgaagatggc tttttcttca ccgttgatcg
      481 taatgctggt agctttcagt gcaaagccca gcgtatcgcg ggtattacgt tggtaggtct
      541 gcgcacccac gccgaagacg atgttactag aggcaaaacc tttcgcttgc aggccttcca
      601 ggattttgaa cattttttcg tacgtaacac cgtcgccata gattgcacca atgtgcggat
      661 tgataacttt atagcctttc tggttcacgg taccgccaaa gatatcccac agacattcaa
      721 tcagaccttt gcgttcatgt tccgtgtcgg cggtcggatc accgcagata atcgcgaaaa
      781 agttgccgct atccgggcga atcaccagac gagcgttttc cggacgtgcg ataatttcct
      841 gtttcagcag cggcagattc acggtgatgt tgtgccaaaa gtccgtggta tcactgacaa
      901 tagacagcat gttatgcggg aatttagcca tcagatagcg aaacgtgctc agttcatcga
      961 cgccgtggct cgacataacg ctatgttccg atgccgggat gctggtacca atcagtgagg
     1021 aactgccgta ataggcttca
//
