LOCUS       sequence-161680-         485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses an shRNA targeting both S6K1 and S6K2 (mouse).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Hartman NW, Lin TV, Zhang L, Paquelet GE, Feliciano DM, Bordey A
  TITLE     mTORC1 targets the translational repressor 4E-BP2, but not S6 kinase
            1/2, to regulate neural stem cell self-renewal in vivo.
  JOURNAL   Cell Rep. 2013 Oct 31;5(2):433-44. doi: 
            10.1016/j.celrep.2013.09.017. Epub 2013 Oct 17.
  PUBMED    24139800
REFERENCE   2  (bases 1 to 485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.09.017"; journalName: "Cell Rep"; date: 
            "2013-10-31- 31"; volume: "5"; issue: "2"; pages: "433-44"
COMMENT     Sequence Label: pSicoR-shS6K1/2
FEATURES             Location/Qualifiers
     source          1..485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(201..234)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     238..258
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 tctagagatc cgacgcgcca tctctaggcc cgcgccggcc ccctcgcaca gacttgtggg
       61 agaagctcgg ctactcccct gccccggtta atttgcatat aatatttcct agtaactata
      121 gaggcttaat gtgcgataaa agacagataa tctgttcttt ttaatactag ctacatttta
      181 catgataggc ttggatttct ataacttcgt atagcataca ttatacgaag ttataaacag
      241 cacaaaagga aactcaccct aactgtaaag taattgtgtg ttttgagact ataaatatcc
      301 cttggagaaa agccttgttt gctcagtgag agtgccaacc aactcgagtt ggttggcact
      361 ctcactgagt tttttctcga gtactaggat ccattaggcg gccgcgtgga taaccgtatt
      421 accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      481 tatgg
//
