LOCUS       sequence-161681-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Balciunas D, Wangensteen KJ, Wilber A, Bell J, Geurts A, Sivasubbu 
            S, Wang X, Hackett PB, Largaespada DA, McIvor RS, Ekker SC
  TITLE     Harnessing a high cargo-capacity transposon for genetic applications
            in vertebrates.
  JOURNAL   PLoS Genet. 2006 Nov 10;2(11):e169. Epub 2006 Aug 28.
  PUBMED    17096595
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2006-11-10"; volume: "2(11)"; pages: "e169. Epub 
            2006 Aug 28"
COMMENT     Sequence Label: pminiTol2
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(8..19,20..207)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     misc_feature    join(352..514,515..526)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(577..595)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(578..595)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(613..629)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(613..629)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(626..648)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    637..653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(661..667,668..685,686..691))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    706..727
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(844..861)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttgggcccag aggtgtaaag tacttgagta attttacttg attactgtac ttaagtatta
       61 tttttgggga tttttacttt acttgagtac aattaaaaat caatactttt acttttactt
      121 aattacattt ttttagaaaa aaaagtactt tttactcctt acaattttat ttacagtcaa
      181 aaagtactta ttttttggag atcacttcat tctattttcc cttgctatta ccaaaccaat
      241 tgaattgcgc tgatgcccag tttaatttaa atagatctgg ccatctagag cggccgcgcg
      301 cactagtgaa ttccatggat atcaagctta aacaagaatc tctagttttc tttcttgctt
      361 ttacttttac ttccttaata ctcaagtaca attttaatgg agtacttttt tacttttact
      421 caagtaagat tctagccaga tacttttact tttaattgag taaaattttc cctaagtact
      481 tgtactttca cttgagtaaa atttttgagt actttttaca cctctgctcg accatatggg
      541 agagctccca acgcgttgga tgcatagctt gagtattcta tagtgtcacc taaatagctt
      601 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      661 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      721 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      781 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      841 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
//
