LOCUS       sequence-161685-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive TEF1 promoter mediated expression of the UBiC and ARO4*
            genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Williams TC, Peng B, Vickers CE, Nielsen LK
  TITLE     The Saccharomyces cerevisiae pheromone-response is a metabolically 
            active stationary phase for bio-production.
  JOURNAL   Metab Eng Commun. 2016 May 11;3:142-152. doi: 
            10.1016/j.meteno.2016.05.001. eCollection 2016 Dec.
  PUBMED    29468120
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2016-05-11"; volume: "3:142-152. doi"; pages: " 
            10.1016/j.meteno.2016.05.001. eCollection 2016 Dec"
COMMENT     Sequence Label: PHBA02
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(82..329)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     295..313
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     355..371
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     355..371
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgcacgatg
       61 agagtgtaaa ctgcgaagct tgcaaattaa agccttcgag cgtcccaaaa ccttctcaag
      121 caaggttttc agtataatgt tacatgcgta cacgcgtttg tacagaaaaa aaagaaaaat
      181 ttgaaatata aataacgttc ttaatactaa cataactata aaaaaataaa tagggaccta
      241 gacttcaggt tgtctaactc cttccttttc ggttagagcg gatgtggggg gagggcgtga
      301 atgtaagcgt gacataacta attacatgat atcgacaaag gaaaaggggc ctgttctaga
      361 actagtggat cccccgggct atttcttgtt aacttctctt ctttgtctga cagcagcagc
      421 caatttcctc aagacgtctt cagtagtttc ccaacctata caagcatcag tgatggaaac
      481 accatatttc aagccggctt taccttcggc tgggatgcct tggttacctt cgttgatgtt
      541 tgattcaatc atgacaccgg taatggcgtt ttcaccgtta gcgatttgct cacaaacaac
      601 gtcattgacc tttggttggt ttctgaaatc cttattggag ttaccgtgag agtagtcaat
      661 cattagaccg ttggaaccgg caggcaattg agccttagct tctgcaacgg acttagcgtc
      721 gtagttggta ccctttttac cacctcttag aataacgaag cagtgttcgt tacccttagt
      781 agtggtgata gcagcaac
//
