LOCUS       sequence-161687-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive TEF1 promoter mediated expression of the UBiC, ARO4*, 
            and TKL1 genes .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Williams TC, Peng B, Vickers CE, Nielsen LK
  TITLE     The Saccharomyces cerevisiae pheromone-response is a metabolically 
            active stationary phase for bio-production.
  JOURNAL   Metab Eng Commun. 2016 May 11;3:142-152. doi: 
            10.1016/j.meteno.2016.05.001. eCollection 2016 Dec.
  PUBMED    29468120
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2016-05-11"; volume: "3:142-152. doi"; pages: " 
            10.1016/j.meteno.2016.05.001. eCollection 2016 Dec"
COMMENT     Sequence Label: PHBA03
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(71..318)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     284..302
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgacgatga gagtgtaaac
       61 tgcgaagctt gcaaattaaa gccttcgagc gtcccaaaac cttctcaagc aaggttttca
      121 gtataatgtt acatgcgtac acgcgtttgt acagaaaaaa aagaaaaatt tgaaatataa
      181 ataacgttct taatactaac ataactataa aaaaataaat agggacctag acttcaggtt
      241 gtctaactcc ttccttttcg gttagagcgg atgtgggggg agggcgtgaa tgtaagcgtg
      301 acataactaa ttacatgata tcgacaaagg aaaaggggcc tgttctagat tagaaagctt
      361 ttttcaaagg agaaattagc ttgtcaccct tatagaatgc aatggtcttt tgagctcttt
      421 cagcaacacc ttctggggtg aaaccgaaga acttgaagac ttctggtgcc ttaccggagg
      481 caccaaatct gtcaataccg aaggattgat gagcgtattt gccccaacat gtggtagcca
      541 aaacttcaac agacatgatt ggaacgttgt ctggtaagac tgatagtctg tattctaggg
      601 gttgtttgtc aaaagtgaag aaatctggta gagaaacaac acgagccttg atgttctttg
      661 cggccaaagt cttagcagct tcaacactca aagacacttc ggaaccagta gccactaaaa
      721 taatatctgg gttagcaaca tcttgtagta cgtaaccacc cttagaagcg ctttcaatag
      781 agctaccttc caattgtggc aagttttgtc tggacaaagc aatgatactt ggagtatgct
      841 tggattctaa agagttcttg taggcggcag aaacttcgtt accatcagct ggtctccaaa
      901 cttgaatgtt tggtagggat ctgaagtgtg ctaaagtttc aataggttga tgtgttggac
      961 catcttcacc gacaccgata gagtcatgtg tagcaaccca aataactggg tggccagaca
     1021 aagcggacaa tctaacggca ccagcagc
//
