LOCUS       sequence-161691-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of RPL8B promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFP77
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    709..742
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taacatcacc atctaattca accaaaattg ggacaacacc agtgaataat tcttcacctt
       61 tagaggatcc catcttttct ttcagttatc gtgttcactg tattgaaaaa cagatataaa
      121 aaaaaaaaat agtgaatttc aacgtgcgca tcacaatact tatgtcttga taataatatc
      181 gatcccatac tattgtccct ggattttgag atgggaaaag tttcaatgcg ttctatcgta
      241 tagtatgatg tattcaaaaa aaatgcgata attctttagt tgtcggcact tcggaagcca
      301 aaaaaaaaaa aaaaaaaaaa aaagaaaaaa agaaaagggt gtctaagatg tacggatctt
      361 cattgggatg gaaaacaggc aagtaaaaag aatagtaatt attacaatgg agaagataag
      421 ataaagttag cggcaacaaa taaagaactc atcagagttc ttggcttctc tatgagtgaa
      481 agtgtttcaa aggtgttttt ttatgagaca acagtaattc ccctccttca gaacagagtg
      541 tagtgattgg cagtgaagct atccctcttt gccacccaat tttgacggca gttctcatag
      601 catctcaaag caatagcagt gcaaaagtac ataaccgtag gaaggtacgc ggtaggtatt
      661 tgagttcgtt ggtggttatc ctccgcaagg cgtcgagcaa cccttaatat aacttcgtat
      721 aatgtatgct atacgaagtt attaggtcta gagatcccaa tacaacagat cacgtgatct
      781 tttgtaagat gaagttgaag tagtgttgca ccgtgccaat gcaggtggct attagattaa
      841 atatgtgatt tgttctatta agtttcctgt ataattaatg gggagcgctg attctctttt
      901 ggtacgcttc ccatccagca tttctgtatc tttcaccttc aaccttagga tctctaccc
//
