LOCUS       sequence-161692-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of RPL8B promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFP77
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(228..938)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 tgcatgcatt taaatcatta cgaccgagat tcccgggtaa taactgatat aattaaattg
       61 aagctctaat ttgtgagttt agtatacatg catttactta taatacagtt ttttagtttt
      121 gctggccgca tcttctcaaa tatgcttccc agcctgcttt tctgtaacgt tcaccctcta
      181 ccttagcatc ccttcccttt gcaaatagtc ctactagttt aagatctttt gtacaattca
      241 tccataccat gggtaatacc agcagcagta acaaattcta acaagaccat gtggtctctc
      301 ttttcgtttg gatctttgga taaggcagat tgagtggata agtaatggtt gtctggtaac
      361 aagactggac catcaccaat tggagtattt tgttgataat ggtcagctaa ttgaacagaa
      421 ccatcttcaa tgttgtgtct aattttgaag ttaactttga taccattctt ttgtttgtca
      481 gccatgatgt aaacattgtg agagttatag ttgtattcca atttgtgacc taaaatgtta
      541 ccatcttctt taaaatcaat accttttaat tcgattctat taactaaggt atcaccttca
      601 aacttgactt cagctctggt cttgtagtta ccgtcatctt tgaaaaaaat agttctttct
      661 tgaacataac cttctggcat ggcagacttg aaaaagtcat gttgtttcat atgatctggg
      721 tatctcgcaa aacattgaac accataaccg aaagtagtga ctaaggttgg ccatggaact
      781 ggcaatttac cagtagtaca aataaatttt aaggtcaatt taccgtaagt agcatcacct
      841 tcaccttcac cggagacaga aaatttgtga ccattaacat caccatctaa ttcaaccaaa
      901 attgggacaa caccagtgaa taattcttca cctttagagg atcccatctt ttctttcagt
      961 tatcgtgttc actgtattga aaaacagata taaaaaaaaa aaatagtgaa tttcaacgtg
     1021 cgcatcacaa tacttatgtc t
//
