LOCUS       sequence-161702-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of RPL3promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFP80
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    777..810
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttaacatcac catctaattc aaccaaaatt gggacaacac cagtgaataa ttcttcacct
       61 ttagaggatc ccatgattga ttgttgtagt aactgtgttg ttctagagat gacaaaaccg
      121 aaatgaaata atgagtaaag aaagatgagg attgacaaag acaactaaat aaatcgtatt
      181 ttatatatcc aggaagcacg aaagagacaa atgaaaaatt tttttcatgg acgatgatca
      241 ctgaaaaaaa aaaaaaaaaa tggaaaacag ttgtgcgtcg cttcttgaaa aacgtacaaa
      301 acgattacta tattactgtc cggccggtta acctaaaaaa gaatattgcc attcattcca
      361 gtgtgcgtga gtagagttta gtttttgcaa ccaatgactg cgaacaggca gcaagtttgc
      421 gagtgctttc tttcccccta taaagctgtc tgaaatgcgt taatccctcc acctttcctc
      481 ttgctgttgc attaccactg aatttaataa attgtttgca tcatttctgt gttgaggact
      541 taacttcccg gcttagcagc tggtttgttc gcaattatcg agcaattctt gaacgtctac
      601 taaggtttgc acatttcgta tacttacttt agaatgataa cgtaagtggc tgatacgagc
      661 ggcactaaca gaatatacga taaacttcat tcacaaaaaa aagcagcaag tgaaaaagtg
      721 ctattcttgt ttgcctttgc ctacctgtac gtaatggcag tcgagcaacc cttaatataa
      781 cttcgtataa tgtatgctat acgaagttat taggtctaga gatcccaata caacagatca
      841 cgtgatcttt tgtaagatga agttgaagta gtgttgcacc gtgccaatgc aggtggctat
      901 tagattaaat atgtgatttg ttctattaag tttcctgtat aattaatggg gagcgctgat
      961 tctcttttgg tacgcttccc atccagcatt tctgtatctt tcaccttcaa ccttaggatc
     1021 tctacccttg g
//
