LOCUS       sequence-161705-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Negative control for HOP-flash. 7xmut TEAD binding sites with 
            minimal promoter plus luciferase reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Kim NG, Gumbiner BM
  TITLE     Adhesion to fibronectin regulates Hippo signaling via the 
            FAK-Src-PI3K pathway.
  JOURNAL   J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. 
            Epub 2015 Jul 27.
  PUBMED    26216901
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201501025"; journalName: "J Cell Biol"; date: 
            "2015-08-3- 3"; volume: "210"; issue: "3"; pages: "503-15"
COMMENT     Sequence Label: HIP-flash (HOP-flash mutant)
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      355..364
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(427..445)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc
       61 cagaacattt ctctatcgat aggtaccgag ctcggatcca ctagtaacgg ccgccagtgt
      121 gctggaattc gcccttggtg gggagagatg cgagagatgt ccctgtttgt ggtggggaga
      181 gatgcgagag atgtccctgt ttgtggtggg gagagatgcg agagatgtcc ctgtttgtgg
      241 tggggagaga tgcgagagat aagggcgaat tctgcagatt agagggtata taatggatcc
      301 ggtactcgag atctgcgatc taagtaagct tggcattccg gtactgttgg taaagccacc
      361 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga
      421 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
      481 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc
      541 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
      601 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
      661 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt
      721 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
      781 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga
      841 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
      901 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa
//
